dc.contributor.author | Fukumura, Dai | |
dc.contributor.author | Jain, Rakesh K | |
dc.contributor.author | Rowlands, Christopher | |
dc.contributor.author | Park, Demian | |
dc.contributor.author | Bruns, Oliver Thomas | |
dc.contributor.author | Piatkevich, Kiryl | |
dc.contributor.author | Bawendi, Moungi G | |
dc.contributor.author | Boyden, Edward | |
dc.contributor.author | So, Peter T. C. | |
dc.date.accessioned | 2018-01-08T17:03:06Z | |
dc.date.available | 2018-01-08T17:03:06Z | |
dc.date.issued | 2017-05 | |
dc.date.submitted | 2016-11 | |
dc.identifier.issn | 2047-7538 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/113016 | |
dc.description.abstract | Three-photon wide-field depth-resolved excitation is used to overcome some of the limitations in conventional point-scanning two- and three-photon microscopy. Excitation of chromophores as diverse as channelrhodopsins and quantum dots is shown, and a penetration depth of more than 700 μm into fixed scattering brain tissue is achieved, approximately twice as deep as that achieved using two-photon wide-field excitation. Compatibility with live animal experiments is confirmed by imaging the cerebral vasculature of an anesthetized mouse; a complete focal stack was obtained without any evidence of photodamage. As an additional validation of the utility of wide-field three-photon excitation, functional excitation is demonstrated by performing three-photon optogenetic stimulation of cultured mouse hippocampal neurons expressing a channelrhodopsin; action potentials could reliably be excited without causing photodamage. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant NIH-5-P41-EB015871-27) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant DP3-DK101024 01) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant 1-U01-NS090438-01) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (1-R01-HL121386-01A1) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant 1-R01-EY017656-06A1) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant 1R24MH106075) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant 2R01DA029639) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant 1R01MH103910) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant 1R01GM104948) | en_US |
dc.description.sponsorship | National Science Foundation (U.S.) (Grant CBET 1053233) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant 5U54 CA151884-04) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant 9-P41-EB015871-26A1) | en_US |
dc.publisher | Springer Nature | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1038/LSA.2016.255 | en_US |
dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en_US |
dc.source | PMC | en_US |
dc.title | Wide-field three-photon excitation in biological samples | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Rowlands, Christopher J et al. “Wide-Field Three-Photon Excitation in Biological Samples.” Light: Science & Applications 6, 5 (November 2016): e16255 © 2017 The Author(s) | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Chemistry | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Mechanical Engineering | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Media Laboratory | en_US |
dc.contributor.department | McGovern Institute for Brain Research at MIT | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Center for Neurobiological Engineering | en_US |
dc.contributor.mitauthor | Rowlands, Christopher | |
dc.contributor.mitauthor | Park, Demian | |
dc.contributor.mitauthor | Bruns, Oliver Thomas | |
dc.contributor.mitauthor | Piatkevich, Kiryl | |
dc.contributor.mitauthor | Bawendi, Moungi G | |
dc.contributor.mitauthor | Boyden, Edward | |
dc.contributor.mitauthor | So, Peter T. C. | |
dc.relation.journal | Light: Science & Applications | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2018-01-05T20:37:29Z | |
dspace.orderedauthors | Rowlands, Christopher J; Park, Demian; Bruns, Oliver T; Piatkevich, Kiryl D; Fukumura, Dai; Jain, Rakesh K; Bawendi, Moungi G; Boyden, Edward S; So, Peter TC | en_US |
dspace.embargo.terms | N | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-8261-2371 | |
dc.identifier.orcid | https://orcid.org/0000-0002-5738-0126 | |
dc.identifier.orcid | https://orcid.org/0000-0003-2220-4365 | |
dc.identifier.orcid | https://orcid.org/0000-0002-0419-3351 | |
dc.identifier.orcid | https://orcid.org/0000-0003-4698-6488 | |
mit.license | OPEN_ACCESS_POLICY | en_US |