Astrin-SKAP complex reconstitution reveals its kinetochore interaction with microtubule-bound Ndc80
Author(s)Su, Kuan-Chung; Wilson-Kubalek, Elizabeth M; Kern, David Matthew; Monda, Julie Kathryn; Cheeseman, Iain M
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Chromosome segregation requires robust interactions between the macromolecular kinetochore structure and dynamic microtubule polymers. A key outstanding question is how kinetochore-microtubule attachments are modulated to ensure that bi-oriented attachments are selectively stabilized and maintained. The Astrin-SKAP complex localizes preferentially to properly bi-oriented sister kinetochores, representing the final outer kinetochore component recruited prior to anaphase onset. Here, we reconstitute the 4-subunit Astrin-SKAP complex, including a novel MYCBP subunit. Our work demonstrates that the Astrin-SKAP complex contains separable kinetochore localization and microtubule binding domains. In addition, through cross-linking analysis in human cells and biochemical reconstitution, we show that the Astrin-SKAP complex binds synergistically to microtubules with the Ndc80 complex to form an integrated interface. We propose a model in which the Astrin-SKAP complex acts together with the Ndc80 complex to stabilize correctly formed kinetochore-microtubule interactions.
DepartmentMassachusetts Institute of Technology. Department of Biology
eLife Sciences Publications, Ltd
Kern, David M et al. “Astrin-SKAP Complex Reconstitution Reveals Its Kinetochore Interaction with Microtubule-Bound Ndc80.” eLife 6, 2017 (August 2017): e26866 © Kern et al
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