Engineered erythrocytes covalently linked to antigenic peptides can protect against autoimmune disease
Author(s)
Bilate, Angelina M.; Huang, Nai-Jia; Li, Hojun; Patterson, Heide C.; Dougan, Stephanie K.; Maruyama, Takeshi; Pishesha, Novalia; Wibowo, Marsha C; Dhesycka, Rhogerry; Bousbaine, Djenet; Lodish, Harvey F; Ploegh, Hidde; Li, Zeyang,S.M.Massachusetts Institute of Technology.; ... Show more Show less
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Show full item recordAbstract
Current therapies for autoimmune diseases rely on traditional immunosuppressive medications that expose patients to an increased risk of opportunistic infections and other complications. Immunoregulatory interventions that act prophylactically or therapeutically to induce antigen-specific tolerance might overcome these obstacles. Here we use the transpeptidase sortase to covalently attach diseaseassociated autoantigens to genetically engineered and to unmodified red blood cells as a means of inducing antigen-specific tolerance. This approach blunts the contribution to immunity of major subsets of immune effector cells (B cells, CD4⁺ and CD8⁺ T cells) in an antigenspecific manner. Transfusion of red blood cells expressing self-antigen epitopes can alleviate and even prevent signs of disease in experimental autoimmune encephalomyelitis, as well as maintain normoglycemia in a mouse model of type 1 diabetes.
Date issued
2017-03Department
Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of BiologyJournal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Pishesha, Novalia et al. “Engineered Erythrocytes Covalently Linked to Antigenic Peptides Can Protect Against Autoimmune Disease.” Proceedings of the National Academy of Sciences 114, 12 (March 2017): 3157–3162 © 2017 National Academy of Sciences
Version: Final published version
ISSN
0027-8424
1091-6490