Optical visualization and quantification of enzyme activity using dynamic droplet lenses
Author(s)
Zarzar, Lauren D.; Kalow, Julia Ann; He, Xinping; Walish, Joseph John; Swager, Timothy M
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In this paper, we describe an approach to measuring enzyme activity based on the reconfiguration of complex emulsions. Changes in the morphology of these complex emulsions, driven by enzyme-responsive surfactants, modulate the transmission of light through a sample. Through this method we demonstrate how simple photodetector measurements may be used to monitor enzyme kinetics. This approach is validated by quantitative measurements of enzyme activity for three different classes of enzymes (amylase, lipase, and sulfatase), relying on two distinct mechanisms for coupling droplet morphology to enzyme activity (host–guest interactions with uncaging and molecular cleavage).
Date issued
2017-03Department
Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies; Massachusetts Institute of Technology. Department of ChemistryJournal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Zarzar, Lauren D., et al. “Optical Visualization and Quantification of Enzyme Activity Using Dynamic Droplet Lenses.” Proceedings of the National Academy of Sciences, vol. 114, no. 15, Apr. 2017, pp. 3821–25. © 2018 National Academy of Sciences
Version: Final published version
ISSN
0027-8424
1091-6490