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dc.contributor.authorMargulies, Carrie Marie
dc.contributor.authorChaim, Isaac Alexander
dc.contributor.authorMazumder, Aprotim
dc.contributor.authorCriscione, June
dc.contributor.authorSamson, Leona D
dc.date.accessioned2018-01-22T15:50:44Z
dc.date.available2018-01-22T15:50:44Z
dc.date.issued2017-09
dc.date.submitted2017-05
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/1721.1/113249
dc.description.abstractThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Alkylating agents are ubiquitous in our internal and external environments, causing DNA damage that contributes to mutations and cell death that can result in aging, tissue degeneration and cancer. Repair of methylated DNA bases occurs primarily through the base excision repair (BER) pathway, a multi-enzyme pathway initiated by the alkyladenine DNA glycosylase (Aag, also known as Mpg). Previous work demonstrated that mice treated with the alkylating agent methyl methanesulfonate (MMS) undergo cerebellar degeneration in an Aag-dependent manner, whereby increased BER initiation by Aag causes increased tissue damage that is dependent on activation of poly (ADP-ribose) polymerase 1 (Parp1). Here, we dissect the molecular mechanism of cerebellar granule neuron (CGN) sensitivity to MMS using primary ex vivo neuronal cultures. We first established a high-throughput fluorescent imaging method to assess primary neuron sensitivity to treatment with DNA damaging agents. Next, we verified that the alkylation sensitivity of CGNs is an intrinsic phenotype that accurately recapitulates the in vivo dependency of alkylation-induced CGN cell death on Aag and Parp1 activity. Finally, we show that MMS-induced CGN toxicity is independent of all the cellular events that have previously been associated with Parp-mediated toxicity, including mitochondrial depolarization, AIF translocation, calcium fluxes, and NAD⁺ consumption. We therefore believe that further investigation is needed to adequately describe all varieties of Parp-mediated cell death.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant R01- ES022872)en_US
dc.description.sponsorshipEllison Medical Foundation (Award AG-SS-3046-12)en_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofhttp://dx.doi.org/10.1371/journal.pone.0184619en_US
dc.rightsCreative Commons Attribution 4.0 International Licenseen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_US
dc.sourcePLoSen_US
dc.titleAlkylation induced cerebellar degeneration dependent on Aag and Parp1 does not occur via previously established cell death mechanismsen_US
dc.typeArticleen_US
dc.identifier.citationMargulies, Carrie M. et al. “Alkylation Induced Cerebellar Degeneration Dependent on Aag and Parp1 Does Not Occur via Previously Established Cell Death Mechanisms.” Edited by Robert W Sobol. PLOS ONE 12, 9 (September 2017): e0184619 © 2017 Margulies et alen_US
dc.contributor.departmentDavid H. Koch Institute for Integrative Cancer Research at MITen_US
dc.contributor.departmentMassachusetts Institute of Technology. Center for Environmental Health Sciencesen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.mitauthorMargulies, Carrie Marie
dc.contributor.mitauthorChaim, Isaac Alexander
dc.contributor.mitauthorMazumder, Aprotim
dc.contributor.mitauthorCriscione, June
dc.contributor.mitauthorSamson, Leona D
dc.relation.journalPLOS ONEen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2018-01-19T15:57:45Z
dspace.orderedauthorsMargulies, Carrie M.; Chaim, Isaac Alexander; Mazumder, Aprotim; Criscione, June; Samson, Leona D.en_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-1757-4954
dc.identifier.orcidhttps://orcid.org/0000-0003-1787-046X
dc.identifier.orcidhttps://orcid.org/0000-0002-7112-1454
mit.licensePUBLISHER_CCen_US


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