dc.contributor.author | Pereira, Jose H. | |
dc.contributor.author | McAndrew, Ryan P. | |
dc.contributor.author | Ralston, Corie Y. | |
dc.contributor.author | King, Jonathan A. | |
dc.contributor.author | Adams, Paul D. | |
dc.contributor.author | Sergeeva, Oksana Andrei | |
dc.date.accessioned | 2018-02-13T18:12:09Z | |
dc.date.available | 2018-02-13T18:12:09Z | |
dc.date.issued | 2017-06 | |
dc.date.submitted | 2017-03 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/113627 | |
dc.description.abstract | The human chaperonin TRiC consists of eight non-identical subunits, and its protein-folding activity is critical for cellular health. Misfolded proteins are associated with many human diseases, such as amyloid diseases, cancer, and neuropathies, making TRiC a potential therapeutic target. A detailed structural understanding of its ATP-dependent folding mechanism and substrate recognition is therefore of great importance. Of particular health-related interest is the mutation Histidine 147 to Arginine (H147R) in human TRiC subunit 5 (CCT5), which has been associated with hereditary sensory neuropathy. In this paper, we describe the crystal structures of CCT5 and the CCT5-H147R mutant, which provide important structural information for this vital protein-folding machine in humans. This first X-ray crystallographic study of a single human CCT subunit in the context of a hexadecameric complex can be expanded in the future to the other 7 subunits that form the TRiC complex. | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1038/S41598-017-03825-3 | en_US |
dc.rights | Creative Commons Attribution 4.0 International License | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_US |
dc.title | Structure of the human TRiC/CCT Subunit 5 associated with hereditary sensory neuropathy | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Pereira, Jose H. et al. “Structure of the Human TRiC/CCT Subunit 5 Associated with Hereditary Sensory Neuropathy.” Scientific Reports 7, 1 (June 2017): 3673 © 2017 The Author(s) | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.mitauthor | Sergeeva, Oksana Andrei | |
dc.relation.journal | Scientific Reports | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2018-02-09T17:55:01Z | |
dspace.orderedauthors | Pereira, Jose H.; McAndrew, Ryan P.; Sergeeva, Oksana A.; Ralston, Corie Y.; King, Jonathan A.; Adams, Paul D. | en_US |
dspace.embargo.terms | N | en_US |
mit.license | PUBLISHER_POLICY | en_US |