dc.contributor.author | Hassan, Musa A. | |
dc.contributor.author | Guo-Liang, Chew | |
dc.contributor.author | Meissner, Markus | |
dc.contributor.author | Nicolai Siegel, T. | |
dc.contributor.author | Vasquez, Juan J. | |
dc.contributor.author | Vasquez Ospina, Juan Jose | |
dc.date.accessioned | 2018-03-28T18:44:09Z | |
dc.date.available | 2018-03-28T18:44:09Z | |
dc.date.issued | 2017-12 | |
dc.date.submitted | 2017-07 | |
dc.identifier.issn | 1471-2164 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/114431 | |
dc.description.abstract | Background: The lytic cycle of the protozoan parasite Toxoplasma gondii, which involves a brief sojourn in the extracellular space, is characterized by defined transcriptional profiles. For an obligate intracellular parasite that is shielded from the cytosolic host immune factors by a parasitophorous vacuole, the brief entry into the extracellular space is likely to exert enormous stress. Due to its role in cellular stress response, we hypothesize that translational control plays an important role in regulating gene expression in Toxoplasma during the lytic cycle. Unlike transcriptional profiles, insights into genome-wide translational profiles of Toxoplasma gondii are lacking.
Methods: We have performed genome-wide ribosome profiling, coupled with high throughput RNA sequencing, in intracellular and extracellular Toxoplasma gondii parasites to investigate translational control during the lytic cycle.
Results: Although differences in transcript abundance were mostly mirrored at the translational level, we observed significant differences in the abundance of ribosome footprints between the two parasite stages. Furthermore, our data suggest that mRNA translation in the parasite is potentially regulated by mRNA secondary structure and upstream open reading frames.
Conclusion: We show that most of the Toxoplasma genes that are dysregulated during the lytic cycle are translationally regulated. Keywords: ribosome profiling; RNA-sequencing; translation efficiency; toxoplasma gondii; apicomplexan | en_US |
dc.publisher | BioMed Central | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1186/s12864-017-4362-6 | en_US |
dc.rights | Creative Commons Attribution | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.source | BioMed Central | en_US |
dc.title | Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondii | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Hassan, Musa A. et al. "Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondii." BMC Genomics 18 (December 2017): 961 © 2017 The Author(s) | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
dc.contributor.mitauthor | Vasquez Ospina, Juan Jose | |
dc.relation.journal | BMC Genomics | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2017-12-17T04:53:11Z | |
dc.language.rfc3066 | en | |
dc.rights.holder | The Author(s). | |
dspace.orderedauthors | Hassan, Musa A.; Vasquez, Juan J.; Guo-Liang, Chew; Meissner, Markus; Nicolai Siegel, T. | en_US |
dspace.embargo.terms | N | en_US |
mit.license | PUBLISHER_CC | en_US |