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dc.contributor.authorHassan, Musa A.
dc.contributor.authorGuo-Liang, Chew
dc.contributor.authorMeissner, Markus
dc.contributor.authorNicolai Siegel, T.
dc.contributor.authorVasquez, Juan J.
dc.contributor.authorVasquez Ospina, Juan Jose
dc.date.accessioned2018-03-28T18:44:09Z
dc.date.available2018-03-28T18:44:09Z
dc.date.issued2017-12
dc.date.submitted2017-07
dc.identifier.issn1471-2164
dc.identifier.urihttp://hdl.handle.net/1721.1/114431
dc.description.abstractBackground: The lytic cycle of the protozoan parasite Toxoplasma gondii, which involves a brief sojourn in the extracellular space, is characterized by defined transcriptional profiles. For an obligate intracellular parasite that is shielded from the cytosolic host immune factors by a parasitophorous vacuole, the brief entry into the extracellular space is likely to exert enormous stress. Due to its role in cellular stress response, we hypothesize that translational control plays an important role in regulating gene expression in Toxoplasma during the lytic cycle. Unlike transcriptional profiles, insights into genome-wide translational profiles of Toxoplasma gondii are lacking. Methods: We have performed genome-wide ribosome profiling, coupled with high throughput RNA sequencing, in intracellular and extracellular Toxoplasma gondii parasites to investigate translational control during the lytic cycle. Results: Although differences in transcript abundance were mostly mirrored at the translational level, we observed significant differences in the abundance of ribosome footprints between the two parasite stages. Furthermore, our data suggest that mRNA translation in the parasite is potentially regulated by mRNA secondary structure and upstream open reading frames. Conclusion: We show that most of the Toxoplasma genes that are dysregulated during the lytic cycle are translationally regulated. Keywords: ribosome profiling; RNA-sequencing; translation efficiency; toxoplasma gondii; apicomplexanen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofhttp://dx.doi.org/10.1186/s12864-017-4362-6en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceBioMed Centralen_US
dc.titleComparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondiien_US
dc.typeArticleen_US
dc.identifier.citationHassan, Musa A. et al. "Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondii." BMC Genomics 18 (December 2017): 961 © 2017 The Author(s)en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.mitauthorVasquez Ospina, Juan Jose
dc.relation.journalBMC Genomicsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2017-12-17T04:53:11Z
dc.language.rfc3066en
dc.rights.holderThe Author(s).
dspace.orderedauthorsHassan, Musa A.; Vasquez, Juan J.; Guo-Liang, Chew; Meissner, Markus; Nicolai Siegel, T.en_US
dspace.embargo.termsNen_US
mit.licensePUBLISHER_CCen_US


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