Detection of Copy Number Alterations Using Single Cell Sequencing
Author(s)Knouse, Kristin Ann; Hendricks, Austin A; Wu, Jie
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Detection of genomic changes at single cell resolution is important for characterizing genetic heterogeneity and evolution in normal tissues, cancers, and microbial populations. Traditional methods for assessing genetic heterogeneity have been limited by low resolution, low sensitivity, and/or low specificity. Single cell sequencing has emerged as a powerful tool for detecting genetic heterogeneity with high resolution, high sensitivity and, when appropriately analyzed, high specificity. Her e we provide a protocol for the isolation, whole genome amplification, sequencing, and analysis of single cells. Our approach allows for the reliable identification of megabase-scale copy number variants in single cells. However, aspects of this protocol can also be applied to investigate other types of genetic alterations in single cells.
DepartmentMassachusetts Institute of Technology. Department of Biology; Koch Institute for Integrative Cancer Research at MIT
Journal of Visualized Experiments
Knouse, Kristin A. et al. “Detection of Copy Number Alterations Using Single Cell Sequencing.” Journal of Visualized Experiments 120 (February 2017): e55143 © 2017 Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License
Final published version