A structural phylogeny for understanding 2-oxoacid oxidoreductase function
Author(s)Gibson, Marcus Ian; Chen, Yang-Ting; Drennan, Catherine L.
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2-Oxoacid:ferredoxin oxidoreductases (OFORs) are essential enzymes in microbial one-carbon metabolism. They use thiamine pyrophosphate to reversibly cleave carbon-carbon bonds, generating low potential (~-500 mV) electrons. Crystallographic analysis of a recently discovered OFOR, an oxalate oxidoreductase (OOR), has provided a second view of OFOR architecture and active site composition. Using these recent structural data along with the previously determined structures of pyruvate:ferredoxin oxidoreductase, structure-function relationships in this superfamily have been expanded and re-evaluated. Additionally, structural motifs have been defined that better serve to distinguish one OFOR subfamily from another and potentially uncover novel OFORs.
DepartmentMassachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Chemistry
Current Opinion in Structural Biology
Gibson, Marcus I et al. “A Structural Phylogeny for Understanding 2-Oxoacid Oxidoreductase Function.” Current Opinion in Structural Biology 41 (December 2016): 54–61 © 2016 Elsevier Ltd
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