Environment Impacts the Metabolic Dependencies of Ras-Driven Non-Small Cell Lung Cancer
Author(s)Papagiannakopoulos, Thales; Olenchock, Benjamin A.; Heyman, Julia E.; Jha, Abhishek K.; Pierce, Kerry A.; Mott, Bryan T.; Clish, Clary B.; Davidson, Shawn M.; Luengo, Alba; Bauer, Matthew R.; Keibler, Mark Andrew; O'Brien, James P.; Gui, Dan Yi; Sullivan, Lucas Bryan; Wasylenko, Thomas Michael; Subbaraj, Lakshmipriya; Chin, Christopher R.; Stephanopolous, Gregory; Jacks, Tyler E.; Vander Heiden, Matthew G.; ... Show more Show less
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Cultured cells convert glucose to lactate, and glutamine is the major source of tricarboxylic acid (TCA)-cycle carbon, but whether the same metabolic phenotype is found in tumors is less studied. We infused mice with lung cancers with isotope-labeled glucose or glutamine and compared the fate of these nutrients in tumor and normal tissue. As expected, lung tumors exhibit increased lactate production from glucose. However, glutamine utilization by both lung tumors and normal lung was minimal, with lung tumors showing increased glucose contribution to the TCA cycle relative to normal lung tissue. Deletion of enzymes involved in glucose oxidation demonstrates that glucose carbon contribution to the TCA cycle is required for tumor formation. These data suggest that understanding nutrient utilization by tumors can predict metabolic dependencies of cancers in vivo. Furthermore, these data argue that the in vivo environment is an important determinant of the metabolic phenotype of cancer cells.
DepartmentDavid H. Koch Institute for Integrative Cancer Research at MIT; Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Chemical Engineering
Davidson, Shawn M. et al. “Environment Impacts the Metabolic Dependencies of Ras-Driven Non-Small Cell Lung Cancer.” Cell Metabolism 23, 3 (March 2016): 517–528 © 2016 Elsevier Inc
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