Producing and Concentrating Lenti-Cre for Mouse Infections
Author(s)Gierut, J. J.; Haigis, K. M.; Jacks, Tyler E.
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Lentiviral vectors offer versatility as vehicles for gene delivery. They can transduce a wide range of cell types and integrate into the host genome, which results in long-term expression of the transgene (Cre) both in vitro and in vivo. This protocol describes how lentiviral particles are produced, purified, and concentrated.
DepartmentKoch Institute for Integrative Cancer Research at MIT
Cold Spring Harbor Protocols
Cold Spring Harbor Laboratory Press
Gierut, J. J. et al. “Producing and Concentrating Lenti-Cre for Mouse Infections.” Cold Spring Harbor Protocols 2014, 3 (March 2014): 304-306 © 2014 Cold Spring Harbor Laboratory Press
Author's final manuscript