Producing and Concentrating Lenti-Cre for Mouse Infections
Author(s)
Gierut, J. J.; Haigis, K. M.; Jacks, Tyler E.
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Lentiviral vectors offer versatility as vehicles for gene delivery. They can transduce a wide range of cell types and integrate into the host genome, which results in long-term expression of the transgene (Cre) both in vitro and in vivo. This protocol describes how lentiviral particles are produced, purified, and concentrated.
Date issued
2014-03Department
Koch Institute for Integrative Cancer Research at MITJournal
Cold Spring Harbor Protocols
Publisher
Cold Spring Harbor Laboratory Press
Citation
Gierut, J. J. et al. “Producing and Concentrating Lenti-Cre for Mouse Infections.” Cold Spring Harbor Protocols 2014, 3 (March 2014): 304-306 © 2014 Cold Spring Harbor Laboratory Press
Version: Author's final manuscript
ISSN
1559-6095