In Vivo Delivery of Lenti-Cre or Adeno-Cre into Mice Using Intranasal Instillation
Author(s)Gierut, J. J.; Haigis, K. M.; Jacks, Tyler E.
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Lung cancer remains the leading cause of cancer deaths among both men and women, with a lower rate of survival than both breast and prostate cancer. Development of the Cre/lox system and improved mouse models have allowed researchers to gain a better understanding of human disease, including lung cancer. Through the viral delivery of Cre, gene function in adult mice can be precisely studied at a specific developmental stage or in a specific cell/tissue type of choice. This protocol describes how to produce adenovirus-Cre precipitate. Using this adeno-Cre (or lentivirus-Cre), Cre can be expressed in mouse lungs. The virus is delivered by intranasal instillation.
DepartmentDavid H. Koch Institute for Integrative Cancer Research at MIT; Massachusetts Institute of Technology. Department of Biology
Cold Spring Harbor Protocols
Cold Spring Harbor Laboratory Press
Gierut, J. J. et al. “In Vivo Delivery of Lenti-Cre or Adeno-Cre into Mice Using Intranasal Instillation.” Cold Spring Harbor Protocols 2014, 3 (March 2014): 307-309 © 2014 Cold Spring Harbor Laboratory Press
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