De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds
Author(s)Dudchenko, Olga; Batra, Sanjit S.; Omer, Arina D.; Hoeger, Marie; Durand, Neva C.; Shamim, Muhammad S.; Machol, Ido; Aiden, Aviva Presser; Aiden, Erez Lieberman; Nyquist, Sarah Kate; Lander, Eric Steven; ... Show more Show less
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The Zika outbreak, spread by the Aedes aegypti mosquito, highlights the need to create high-quality assemblies of large genomes in a rapid and cost-effective way. Here we combine Hi-C data with existing draft assemblies to generate chromosome-length scaffolds. We validate this method by assembling a human genome, de novo, from short reads alone (67× coverage). We then combine our method with draft sequences to create genome assemblies of the mosquito disease vectors Ae. aegypti and Culex quinquefasciatus, each consisting of three scaffolds corresponding to the three chromosomes in each species. These assemblies indicate that almost all genomic rearrangements among these species occur within, rather than between, chromosome arms. The genome assembly procedure we describe is fast, inexpensive, and accurate, and can be applied to many species.
DepartmentMassachusetts Institute of Technology. Computational and Systems Biology Program; Massachusetts Institute of Technology. Department of Biology
American Association for the Advancement of Science (AAAS)
Dudchenko, Olga, Sanjit S. Batra, Arina D. Omer, Sarah K. Nyquist, Marie Hoeger, Neva C. Durand, Muhammad S. Shamim, et al. “De Novo Assembly of the Aedes Aegypti Genome Using Hi-C Yields Chromosome-Length Scaffolds.” Science 356, no. 6333 (March 23, 2017): 92–95.
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