Show simple item record

dc.contributor.authordiCenzo, George C.
dc.contributor.authorBenedict, Alex B.
dc.contributor.authorFondi, Marco
dc.contributor.authorFinan, Turlough M.
dc.contributor.authorMengoni, Alessio
dc.contributor.authorWalker, Graham C
dc.contributor.authorGriffitts, Joel S
dc.date.accessioned2018-07-12T17:13:27Z
dc.date.available2018-07-12T17:13:27Z
dc.date.issued2018-04
dc.date.submitted2018-02
dc.identifier.issn1553-7404
dc.identifier.urihttp://hdl.handle.net/1721.1/116938
dc.description.abstractBacterial genome evolution is characterized by gains, losses, and rearrangements of functional genetic segments. The extent to which large-scale genomic alterations influence genotype-phenotype relationships has not been investigated in a high-throughput manner. In the symbiotic soil bacterium Sinorhizobium meliloti, the genome is composed of a chromosome and two large extrachromosomal replicons (pSymA and pSymB, which together constitute 45% of the genome). Massively parallel transposon insertion sequencing (Tn-seq) was employed to evaluate the contributions of chromosomal genes to growth fitness in both the presence and absence of these extrachromosomal replicons. Ten percent of chromosomal genes from diverse functional categories are shown to genetically interact with pSymA and pSymB. These results demonstrate the pervasive robustness provided by the extrachromosomal replicons, which is further supported by constraint-based metabolic modeling. A comprehensive picture of core S. meliloti metabolism was generated through a Tn-seq-guided in silico metabolic network reconstruction, producing a core network encompassing 726 genes. This integrated approach facilitated functional assignments for previously uncharacterized genes, while also revealing that Tn-seq alone missed over a quarter of wild-type metabolism. This work highlights the many functional dependencies and epistatic relationships that may arise between bacterial replicons and across a genome, while also demonstrating how Tn-seq and metabolic modeling can be used together to yield insights not obtainable by either method alone.en_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofhttp://dx.doi.org/10.1371/journal.pgen.1007357en_US
dc.rightsCreative Commons Attribution 4.0 International Licenseen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourcePLoSen_US
dc.titleRobustness encoded across essential and accessory replicons of the ecologically versatile bacterium Sinorhizobium melilotien_US
dc.typeArticleen_US
dc.identifier.citationdiCenzo, George C., Alex B. Benedict, Marco Fondi, Graham C. Walker, Turlough M. Finan, Alessio Mengoni, and Joel S. Griffitts. “Robustness Encoded Across Essential and Accessory Replicons of the Ecologically Versatile Bacterium Sinorhizobium Meliloti.” Edited by Josep Casadesús. PLOS Genetics 14, no. 4 (April 19, 2018): e1007357.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.mitauthorWalker, Graham C
dc.contributor.mitauthorGriffitts, Joel S
dc.relation.journalPLOS Geneticsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2018-07-12T13:22:34Z
dspace.orderedauthorsdiCenzo, George C.; Benedict, Alex B.; Fondi, Marco; Walker, Graham C.; Finan, Turlough M.; Mengoni, Alessio; Griffitts, Joel S.en_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0001-7243-8261
mit.licensePUBLISHER_CCen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record