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dc.contributor.authorCheng, Bo
dc.contributor.authorLi, Tiandao
dc.contributor.authorRahl, Peter B.
dc.contributor.authorAdamson, Todd E.
dc.contributor.authorLoudas, Nicholas B.
dc.contributor.authorGuo, Jiannan
dc.contributor.authorVarzavand, Katayoun
dc.contributor.authorCooper, Jeffrey J.
dc.contributor.authorHu, Xiaopeng
dc.contributor.authorGnatt, Averell
dc.contributor.authorPrice, David H.
dc.contributor.authorYoung, Richard A
dc.date.accessioned2018-07-13T18:01:11Z
dc.date.available2018-07-13T18:01:11Z
dc.date.issued2012-01
dc.date.submitted2011-08
dc.identifier.issn1097-2765
dc.identifier.issn1097-4164
dc.identifier.urihttp://hdl.handle.net/1721.1/116987
dc.description.abstractMost human genes are loaded with promoter-proximally paused RNA polymerase II (Pol II) molecules that are poised for release into productive elongation by P-TEFb. We present evidence that Gdown1, the product of the POLR2M gene that renders Pol II responsive to Mediator, is involved in Pol II elongation control. During in vitro transcription, Gdown1 specifically blocked elongation stimulation by TFIIF, inhibited the termination activity of TTF2, and influenced pausing factors NELF and DSIF, but did not affect the function of TFIIS or the mRNA capping enzyme. Without P-TEFb, Gdown1 led to the production of stably paused polymerases in the presence of nuclear extract. Supporting these mechanistic insights, ChIP-Seq demonstrated that Gdown1 mapped over essentially all poised polymerases across the human genome. Our results establish that Gdown1 stabilizes poised polymerases while maintaining their responsiveness to P-TEFb and suggest that Mediator overcomes a Gdown1-mediated block of initiation by allowing TFIIF function.en_US
dc.description.sponsorshipNational Human Genome Research Institute (U.S.) (Grant HG002668-05)en_US
dc.publisherElsevieren_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.molcel.2011.10.022en_US
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs Licenseen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.sourcePMCen_US
dc.titleFunctional Association of Gdown1 with RNA Polymerase II Poised on Human Genesen_US
dc.typeArticleen_US
dc.identifier.citationCheng, Bo et al. “Functional Association of Gdown1 with RNA Polymerase II Poised on Human Genes.” Molecular Cell 45, 1 (January 2012): 38–50 © 2012 Elsevier Incen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.mitauthorYoung, Richard A
dc.relation.journalMolecular Cellen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2018-07-13T17:41:19Z
dspace.orderedauthorsCheng, Bo; Li, Tiandao; Rahl, Peter B.; Adamson, Todd E.; Loudas, Nicholas B.; Guo, Jiannan; Varzavand, Katayoun; Cooper, Jeffrey J.; Hu, Xiaopeng; Gnatt, Averell; Young, Richard A.; Price, David H.en_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0001-8855-8647
mit.licensePUBLISHER_CCen_US


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