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dc.contributor.authorOdstrcil, Iris
dc.contributor.authorFörster, Dominique
dc.contributor.authorRamirez, Alyson
dc.contributor.authorGagnon, James A.
dc.contributor.authorRandlett, Owen
dc.contributor.authorSchoppik, David
dc.contributor.authorEngert, Florian
dc.contributor.authorBaier, Herwig
dc.contributor.authorFreifeld, Limor
dc.contributor.authorCosta, Emma K.
dc.contributor.authorAsano, Shoh M
dc.contributor.authorCeliker, Orhan Tunc
dc.contributor.authorGao, Ruixuan
dc.contributor.authorMartin Alarcon, Daniel Alberto
dc.contributor.authorReginato, Paul Louis
dc.contributor.authorDick, Cortni D.
dc.contributor.authorChen, Linlin
dc.contributor.authorBoyden, Edward
dc.date.accessioned2018-07-24T17:16:56Z
dc.date.available2018-07-24T17:16:56Z
dc.date.issued2017-12
dc.date.submitted2017-04
dc.identifier.issn0027-8424
dc.identifier.issn1091-6490
dc.identifier.urihttp://hdl.handle.net/1721.1/117082
dc.description.abstractExpansion microscopy (ExM) allows scalable imaging of preserved 3D biological specimens with nanoscale resolution on fast diffraction-limited microscopes. Here, we explore the utility of ExM in the larval and embryonic zebrafish, an important model organism for the study of neuroscience and development. Regarding neuroscience, we found that ExM enabled the tracing of fine processes of radial glia, which are not resolvable with diffraction-limited microscopy. ExM further resolved putative synaptic connections, as well as molecular differences between densely packed synapses. Finally, ExM could resolve subsynaptic protein organization, such as ring-like structures composed of glycine receptors. Regarding development, we used ExM to characterize the shapes of nuclear invaginations and channels, and to visualize cytoskeletal proteins nearby. We detected nuclear invagination channels at late prophase and telophase, potentially suggesting roles for such channels in cell division. Thus, ExM of the larval and embryonic zebrafish may enable systematic studies of how molecular components are configured in multiple contexts of interest to neuroscience and developmental biology.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant 1R01EB024261)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant 1R01MH110932)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant 2R01DA029639)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant 1R01NS087950)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant 1U01MH106011)en_US
dc.publisherNational Academy of Sciences (U.S.)en_US
dc.relation.isversionofhttp://dx.doi.org/10.1073/PNAS.1706281114en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePNASen_US
dc.titleExpansion microscopy of zebrafish for neuroscience and developmental biology studiesen_US
dc.typeArticleen_US
dc.identifier.citationFreifeld, Limor et al. “Expansion Microscopy of Zebrafish for Neuroscience and Developmental Biology Studies.” Proceedings of the National Academy of Sciences 114, 50 (November 2017): E10799–E10808 © 2017 the Author(s)en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Brain and Cognitive Sciencesen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Scienceen_US
dc.contributor.departmentMassachusetts Institute of Technology. Media Laboratoryen_US
dc.contributor.departmentMcGovern Institute for Brain Research at MITen_US
dc.contributor.mitauthorFreifeld, Limor
dc.contributor.mitauthorCosta, Emma K.
dc.contributor.mitauthorAsano, Shoh M
dc.contributor.mitauthorCeliker, Orhan Tunc
dc.contributor.mitauthorGao, Ruixuan
dc.contributor.mitauthorMartin Alarcon, Daniel Alberto
dc.contributor.mitauthorReginato, Paul Louis
dc.contributor.mitauthorDick, Cortni D.
dc.contributor.mitauthorChen, Linlin
dc.contributor.mitauthorBoyden, Edward
dc.relation.journalProceedings of the National Academy of Sciencesen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2018-07-18T18:22:36Z
dspace.orderedauthorsFreifeld, Limor; Odstrcil, Iris; Förster, Dominique; Ramirez, Alyson; Gagnon, James A.; Randlett, Owen; Costa, Emma K.; Asano, Shoh; Celiker, Orhan T.; Gao, Ruixuan; Martin-Alarcon, Daniel A.; Reginato, Paul; Dick, Cortni; Chen, Linlin; Schoppik, David; Engert, Florian; Baier, Herwig; Boyden, Edward S.en_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-0739-6947
dc.identifier.orcidhttps://orcid.org/0000-0002-5044-0296
dc.identifier.orcidhttps://orcid.org/0000-0003-0547-8129
dc.identifier.orcidhttps://orcid.org/0000-0002-7525-9047
dc.identifier.orcidhttps://orcid.org/0000-0002-0419-3351
mit.licensePUBLISHER_POLICYen_US


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