CNS disease models with human pluripotent stem cells in the CRISPR age
Author(s)Muffat, Julien; Lin, Yun; Jaenisch, Rudolf
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In vitro differentiation of human pluripotent stem cells provides a systematic platform to investigate the physiological development and function of the human nervous system, as well as the etiology and consequence when these processes go awry. Recent development in three-dimensional (3D) organotypic culture systems allows modeling of the complex structure formation of the human CNS, and the intricate interactions between various resident neuronal and glial cell types. Combined with an ever-expanding genome editing and regulation toolkit such as CRISPR/Cas9, it is now a possibility to study human neurological disease in the relevant molecular, cellular and anatomical context. In this article, we review recent progress in 3D neural culture and the implications for disease modeling.
DepartmentDavid H. Koch Institute for Integrative Cancer Research at MIT; Massachusetts Institute of Technology. Department of Biology
Current Opinion in Cell Biology
Muffat, Julien et al. “CNS Disease Models with Human Pluripotent Stem Cells in the CRISPR Age.” Current Opinion in Cell Biology 43 (December 2016): 96–103 © 2016 Published by Elsevier Ltd
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