Nitrogen cost minimization is promoted by structural changes in the transcriptome of N-deprived Prochlorococcus cells
Author(s)Read, Robert W; Neveux, Iva; Grzymski, Joseph J; Berube, Paul M.; Biller, Steven; Cubillos, Andres Fernando; Chisholm, Sallie (Penny); ... Show more Show less
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Prochlorococcus is a globally abundant marine cyanobacterium with many adaptations that reduce cellular nutrient requirements, facilitating growth in its nutrient-poor environment. One such genomic adaptation is the preferential utilization of amino acids containing fewer N-atoms, which minimizes cellular nitrogen requirements. We predicted that transcriptional regulation might further reduce cellular N budgets during transient N limitation. To explore this, we compared transcription start sites (TSSs) in Prochlorococcus MED4 under N-deprived and N-replete conditions. Of 64 genes with primary and internal TSSs in both conditions, N-deprived cells initiated transcription downstream of primary TSSs more frequently than N-replete cells. Additionally, 117 genes with only an internal TSS demonstrated increased internal transcription under N-deprivation. These shortened transcripts encode predicted proteins with an average of 21% less N content compared to full-length transcripts. We hypothesized that low translation rates, which afford greater control over protein abundances, would be beneficial to relatively slow-growing organisms like Prochlorococcus. Consistent with this idea, we found that Prochlorococcus exhibits greater usage of glycine-glycine motifs, which causes translational pausing, when compared to faster growing microbes. Our findings indicate that structural changes occur within the Prochlorococcus MED4 transcriptome during N-deprivation, potentially altering the size and structure of proteins expressed under nutrient limitation.
DepartmentMassachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Civil and Environmental Engineering; Massachusetts Institute of Technology. Microbiology Graduate Program
Nature Publishing Group
Read, Robert W et al. “Nitrogen Cost Minimization Is Promoted by Structural Changes in the Transcriptome of N-Deprived Prochlorococcus Cells.” The ISME Journal 11, 10 (June 2017): 2267–2278 © 2017 International Society for Microbial Ecology
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