Adult-onset, chronic, cyclic thrombocytopenia in a Rhesus macaque (
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Author(s)
Metcalf Pate, Kelly A.
de Laforcade, Armelle M.
Bosch, Irene
Lyons, Claire E.
Tompkins, Ronald G.
Frydman, Galit
Marini, Robert P
Bakthavatchalu, Vasudevan
Muthupalani, Sureshkumar
Swennes, Alton G.
Date Issued
May 2017
Journal
Veterinary Clinical Pathology
Publisher
Wiley Blackwell
Citation
Frydman, Galit H. et al. “Adult-Onset, Chronic, Cyclic Thrombocytopenia in a Rhesus Macaque (Macaca Mulatta) after Dengue Virus Vaccination and Viral Challenge.” Veterinary Clinical Pathology 46, 2 (May 2017): 238–247 © 2017 American Society for Veterinary Clinical Pathology
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Author's final manuscript
Abstract
An 8-year-old, male Rhesus macaque (Macaca mulatta), previously used for dengue virus (DENV) vaccine research with viral challenge, was presented with adult-onset, chronic, cyclic thrombocytopenia. Platelet number, morphology, and function were evaluated by automated hematology, peripheral blood smears, electron microscopy, flow cytometry, and impedance aggregometry. Bone marrow was evaluated by cytology. Both serum anti-dengue nonstructural protein 1 (NS1) antibodies and anti-platelet antibodies were detected by ELISA. Platelet characterization showed a lack of aggregation to all agonists (ADP, ASP, and collagen), increased activation with increased expression of surface marker (HLA-ABC), and an absence of surface receptor GPIX during clinical episodes of petechiae and ecchymoses, even in the presence of normal platelet counts. Bone marrow aspirates identified potential mild megakaryocytic hypoplasia. All platelet functions and morphologic attributes were within normal limits during clinically normal phases. Presence of anti-dengue NS1 serum antibodies confirmed a positive DENV titer 8 years postvaccination. Based on the history and clinical findings, a primary differential diagnosis for this chronic, cyclic platelet pathology was autoimmune platelet destruction with potential bone marrow involvement.
MIT Department
Institute for Medical Engineering and Science
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Division of Comparative Medicine
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DOI of Published Version
https://doi.org/10.1111/VCP.12497
