dc.contributor.author | Andrews, James Owen | |
dc.contributor.author | Conway, W. | |
dc.contributor.author | Cho, Won-ki | |
dc.contributor.author | Narayanan, Arjun | |
dc.contributor.author | Spille, Jan Hendrik | |
dc.contributor.author | Jayanth, Namrata | |
dc.contributor.author | Inoue, Takuma | |
dc.contributor.author | Thaler, Jesse | |
dc.contributor.author | Cisse, Ibrahim I | |
dc.contributor.author | Mullen, Susan | |
dc.date.accessioned | 2018-10-25T20:08:03Z | |
dc.date.available | 2018-10-25T20:08:03Z | |
dc.date.issued | 2018-05 | |
dc.date.submitted | 2017-12 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/118788 | |
dc.description.abstract | We present qSR, an analytical tool for the quantitative analysis of single molecule based super-resolution data. The software is created as an open-source platform integrating multiple algorithms for rigorous spatial and temporal characterizations of protein clusters in super-resolution data of living cells. First, we illustrate qSR using a sample live cell data of RNA Polymerase II (Pol II) as an example of highly dynamic sub-diffractive clusters. Then we utilize qSR to investigate the organization and dynamics of endogenous RNA Polymerase I (Pol I) in live human cells, throughout the cell cycle. Our analysis reveals a previously uncharacterized transient clustering of Pol I. Both stable and transient populations of Pol I clusters co-exist in individual living cells, and their relative fraction vary during cell cycle, in a manner correlating with global gene expression. Thus, qSR serves to facilitate the study of protein organization and dynamics with very high spatial and temporal resolutions directly in live cell. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) | en_US |
dc.description.sponsorship | National Cancer Institute (U.S.) (NIH Director’s New Innovator Award DP2-CA195769) | en_US |
dc.description.sponsorship | Massachusetts Institute of Technology. Department of Physics | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1038/s41598-018-25454-0 | en_US |
dc.rights | Creative Commons Attribution 4.0 International License | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.source | Nature | en_US |
dc.title | qSR: a quantitative super-resolution analysis tool reveals the cell-cycle dependent organization of RNA Polymerase I in live human cells | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Andrews, J. O., et al. “QSR: A Quantitative Super-Resolution Analysis Tool Reveals the Cell-Cycle Dependent Organization of RNA Polymerase I in Live Human Cells.” Scientific Reports, vol. 8, no. 1, Dec. 2018. © 2018 The Authors | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Physics | en_US |
dc.contributor.mitauthor | Andrews, James Owen | |
dc.contributor.mitauthor | Conway, W. | |
dc.contributor.mitauthor | Cho, Won-ki | |
dc.contributor.mitauthor | Narayanan, Arjun | |
dc.contributor.mitauthor | Spille, Jan Hendrik | |
dc.contributor.mitauthor | Jayanth, Namrata | |
dc.contributor.mitauthor | Inoue, Takuma | |
dc.contributor.mitauthor | Thaler, Jesse | |
dc.contributor.mitauthor | Cisse, Ibrahim I | |
dc.contributor.mitauthor | Mullen, Susan | |
dc.relation.journal | Scientific Reports | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2018-10-11T16:36:05Z | |
dspace.orderedauthors | Andrews, J. O.; Conway, W.; Cho, W -K.; Narayanan, A.; Spille, J -H.; Jayanth, N.; Inoue, T.; Mullen, S.; Thaler, J.; Cissé, I. I. | en_US |
dspace.embargo.terms | N | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-1867-4380 | |
dc.identifier.orcid | https://orcid.org/0000-0001-9336-0686 | |
dc.identifier.orcid | https://orcid.org/0000-0002-2269-3253 | |
dc.identifier.orcid | https://orcid.org/0000-0001-8493-4721 | |
dc.identifier.orcid | https://orcid.org/0000-0001-9746-6007 | |
dc.identifier.orcid | https://orcid.org/0000-0003-0362-0072 | |
dc.identifier.orcid | https://orcid.org/0000-0002-2406-8160 | |
dc.identifier.orcid | https://orcid.org/0000-0002-8764-1809 | |
mit.license | PUBLISHER_CC | en_US |