The etiology of uracil residues in the Saccharomyces cerevisiae genomic DNA
Author(s)
Stokdyk, Kasey; Kim, Nayun; Owiti, Norah Auma
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Non-canonical residue in DNA is a major and conserved source of genome instability. The appearance of uracil residues in DNA accompanies a significant mutagenic consequence and is regulated at multiple levels, from the concentration of available dUTP in the nucleotide pool to the excision repair for removal from DNA. Recently, an interesting phenomenon of transcription-associated elevation in uracil-derived mutations was described in Saccharomyces cerevisiae genome. While trying to understand the variability in mutagenesis, we uncovered that the frequency of uracil incorporation into DNA can vary depending on the transcription rate and that the non-replicative, repair-associated DNA synthesis underlies the higher uracil density of the actively transcribed genomic loci. This novel mechanism brings together the chemical vulnerability of DNA under transcription and the uracil-associated mutagenesis, and has the potential to apply to other non-canonical residues of mutagenic importance. Keyword: DNA repair; Non-canonical nucleotides; Transcription-associated mutagenesis; Uracil dUTPase
Date issued
2018-10Department
Massachusetts Institute of Technology. Department of Biological EngineeringJournal
Current Genetics
Publisher
Springer Berlin Heidelberg
Citation
Owiti, Norah et al. “The Etiology of Uracil Residues in the Saccharomyces Cerevisiae Genomic DNA.” Current Genetics (October 2018): 1-7 © 2018 The Author(s)
Version: Final published version
ISSN
0172-8083
1432-0983