Selective single cell detachment and retrieval for downstream analyses using nanosecond laser pulses in cnt-coated microwell arrays
Author(s)Chen, Yu-Chih; Baac, Hyoung Won; Lee, Kyu-Tae; Teichert, Kendall; Guo, L. Jay; Yoon, Euisik; Hart, Anastasios John; ... Show more Show less
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Cellular heterogeneity is one of the key hallmarks in cancer biology, but conventional dish-based assays only report the average behavior of many cells. Microfluidics can facilitate manipulating and monitoring of individual cells, yet it is difficult to retrieve a specific target single cell from an enclosed microfluidic chip. In this work, we have successfully developed a selective cell detachment and retrieval scheme with a spatial resolution of around 10μm. The retrieved cells were proved to be viable, and the detachment process has negligible effect on membrane proteins and mRNA expression, providing an ideal tools for the downstream analysis of target cells.
DepartmentMassachusetts Institute of Technology. Department of Mechanical Engineering
19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Transducers Research Foundation, Inc.
Chen, Yu-Chih, Hyoung Won Baac, Kyu-Tae Lee, Kendall Teichert, A. John Hart, L. Jay Guo and Euisik Yoon. "Selective single cell detachment and retrieval for downstream analyses using nanosecond laser pulses in cnt-coated microwell arrays." 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, October 25-29, 2015, Gyeongju, Korea.
Author's final manuscript