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dc.contributor.authorAskoxylakis, Vasileios
dc.contributor.authorDatta, Meenal
dc.contributor.authorFukumura, Dai
dc.contributor.authorJain, Rakesh K.
dc.contributor.authorCarr, Jessica Ann
dc.contributor.authorFranke, Daniel
dc.contributor.authorCaram, Justin R
dc.contributor.authorPerkinson, Collin Fisher
dc.contributor.authorSaif, Mari
dc.contributor.authorBawendi, Moungi G
dc.contributor.authorBruns, Oliver Thomas
dc.date.accessioned2018-12-05T16:48:57Z
dc.date.available2018-12-05T16:48:57Z
dc.date.issued2018-04
dc.identifier.issn0027-8424
dc.identifier.issn1091-6490
dc.identifier.urihttp://hdl.handle.net/1721.1/119450
dc.description.abstractFluorescence imaging is a method of real-time molecular tracking in vivo that has enabled many clinical technologies. Imaging in the shortwave IR (SWIR; 1,000–2,000 nm) promises higher contrast, sensitivity, and penetration depths compared with conventional visible and near-IR (NIR) fluorescence imaging. However, adoption of SWIR imaging in clinical settings has been limited, partially due to the absence of US Food and Drug Administration (FDA)-approved fluorophores with peak emission in the SWIR. Here, we show that commercially available NIR dyes, including the FDA-approved contrast agent indocyanine green (ICG), exhibit optical properties suitable for in vivo SWIR fluorescence imaging. Even though their emission spectra peak in the NIR, these dyes outperform commercial SWIR fluorophores and can be imaged in the SWIR, even beyond 1,500 nm. We show real-time fluorescence imaging using ICG at clinically relevant doses, including intravital microscopy, noninvasive imaging in blood and lymph vessels, and imaging of hepatobiliary clearance, and show increased contrast compared with NIR fluorescence imaging. Furthermore, we show tumor-targeted SWIR imaging with IRDye 800CW-labeled trastuzumab, an NIR dye being tested in multiple clinical trials. Our findings suggest that high-contrast SWIR fluorescence imaging can be implemented alongside existing imaging modalities by switching the detection of conventional NIR fluorescence systems from silicon-based NIR cameras to emerging indium gallium arsenide-based SWIR cameras. Using ICG in particular opens the possibility of translating SWIR fluorescence imaging to human clinical applications. Indeed, our findings suggest that emerging SWIR-fluorescent in vivo contrast agents should be benchmarked against the SWIR emission of ICG in blood. Keywords: shortwave infrared; biomedical imaging; fluorescence imaging green; near infrared; indocyanineen_US
dc.description.sponsorshipUnited States. Department of Energy. Office of Basic Energy Sciences (Award DE-FG02-07ER46454)en_US
dc.publisherNational Academy of Sciences (U.S.)en_US
dc.relation.isversionofhttp://dx.doi.org/10.1073/PNAS.1718917115en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePNASen_US
dc.titleShortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine greenen_US
dc.typeArticleen_US
dc.identifier.citationCarr, Jessica A. et al. “Shortwave Infrared Fluorescence Imaging with the Clinically Approved Near-Infrared Dye Indocyanine Green.” Proceedings of the National Academy of Sciences 115, 17 (April 2018): 4465–4470 © 2018 National Academy of Sciencesen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemistryen_US
dc.contributor.mitauthorCarr, Jessica Ann
dc.contributor.mitauthorFranke, Daniel
dc.contributor.mitauthorCaram, Justin R
dc.contributor.mitauthorPerkinson, Collin Fisher
dc.contributor.mitauthorSaif, Mari
dc.contributor.mitauthorBawendi, Moungi G
dc.contributor.mitauthorBruns, Oliver Thomas
dc.relation.journalProceedings of the National Academy of Sciencesen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2018-12-04T15:49:51Z
dspace.orderedauthorsCarr, Jessica A.; Franke, Daniel; Caram, Justin R.; Perkinson, Collin F.; Saif, Mari; Askoxylakis, Vasileios; Datta, Meenal; Fukumura, Dai; Jain, Rakesh K.; Bawendi, Moungi G.; Bruns, Oliver T.en_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0001-8637-8108
dc.identifier.orcidhttps://orcid.org/0000-0001-6990-4372
dc.identifier.orcidhttps://orcid.org/0000-0003-1192-4746
dc.identifier.orcidhttps://orcid.org/0000-0002-5676-1998
dc.identifier.orcidhttps://orcid.org/0000-0003-2220-4365
dc.identifier.orcidhttps://orcid.org/0000-0002-5738-0126
mit.licensePUBLISHER_POLICYen_US


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