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dc.contributor.authorPuram, Sidharth V.
dc.contributor.authorTirosh, Itay
dc.contributor.authorParikh, Anuraag S.
dc.contributor.authorPatel, Anoop P.
dc.contributor.authorYizhak, Keren
dc.contributor.authorGillespie, Shawn
dc.contributor.authorRodman, Christopher
dc.contributor.authorLuo, Christina L.
dc.contributor.authorMroz, Edmund A.
dc.contributor.authorEmerick, Kevin S.
dc.contributor.authorDeschler, Daniel G.
dc.contributor.authorVarvares, Mark A.
dc.contributor.authorMylvaganam, Ravi
dc.contributor.authorRozenblatt-Rosen, Orit
dc.contributor.authorRocco, James W.
dc.contributor.authorFaquin, William C.
dc.contributor.authorLin, Derrick T.
dc.contributor.authorRegev, Aviv
dc.contributor.authorBernstein, Bradley E.
dc.date.accessioned2018-12-21T20:45:42Z
dc.date.available2018-12-21T20:45:42Z
dc.date.issued2017-11
dc.date.submitted2017-08
dc.identifier.issn0962-8924
dc.identifier.urihttp://hdl.handle.net/1721.1/119831
dc.description.abstractThe diverse malignant, stromal, and immune cells in tumors affect growth, metastasis, and response to therapy. We profiled transcriptomes of ∼6,000 single cells from 18 head and neck squamous cell carcinoma (HNSCC) patients, including five matched pairs of primary tumors and lymph node metastases. Stromal and immune cells had consistent expression programs across patients. Conversely, malignant cells varied within and between tumors in their expression of signatures related to cell cycle, stress, hypoxia, epithelial differentiation, and partial epithelial-to-mesenchymal transition (p-EMT). Cells expressing the p-EMT program spatially localized to the leading edge of primary tumors. By integrating single-cell transcriptomes with bulk expression profiles for hundreds of tumors, we refined HNSCC subtypes by their malignant and stromal composition and established p-EMT as an independent predictor of nodal metastasis, grade, and adverse pathologic features. Our results provide insight into the HNSCC ecosystem and define stromal interactions and a p-EMT program associated with metastasis. Single-cell transcriptomic analysis in patients with head and neck squamous cell carcinoma highlights the heterogeneous composition of malignant and non-malignant cells in the tumor microenvironment and associates a partial EMT program with metastasis. Keywords: single-cell RNA sequencing; metastasis; head and neck squamous cell carcinoma; epithelial-to-mesenchymal transition; tumor microenvironment; scRNA-seq; intra-tumoral heterogeneityen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/J.CELL.2017.10.044en_US
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs Licenseen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.sourcePMCen_US
dc.titleSingle-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Head and Neck Canceren_US
dc.typeArticleen_US
dc.identifier.citationPuram, Sidharth V. et al. “Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Head and Neck Cancer.” Cell 171, 7 (December 2017): 1611–1624 © 2017 Elsevier Incen_US
dc.contributor.departmentDavid H. Koch Institute for Integrative Cancer Research at MITen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.mitauthorRegev, Aviv
dc.relation.journalCellen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2018-12-19T16:12:41Z
dspace.orderedauthorsPuram, Sidharth V.; Tirosh, Itay; Parikh, Anuraag S.; Patel, Anoop P.; Yizhak, Keren; Gillespie, Shawn; Rodman, Christopher; Luo, Christina L.; Mroz, Edmund A.; Emerick, Kevin S.; Deschler, Daniel G.; Varvares, Mark A.; Mylvaganam, Ravi; Rozenblatt-Rosen, Orit; Rocco, James W.; Faquin, William C.; Lin, Derrick T.; Regev, Aviv; Bernstein, Bradley E.en_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0001-8567-2049
mit.licensePUBLISHER_CCen_US


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