Tomographic phase microscopy: principles and applications in bioimaging [Invited]
Author(s)Jin, Di; Zhou, Renjie; Yaqoob, Zahid; So, Peter T. C.
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Tomographic phase microscopy (TPM) is an emerging optical microscopic technique for bioimaging. TPM uses digital holographic measurements of complex scattered fields to reconstruct three-dimensional refractive index (RI) maps of cells with diffraction-limited resolution by solving inverse scattering problems. In this paper, we review the developments of TPM from the fundamental physics to its applications in bioimaging. We first provide a comprehensive description of the tomographic reconstruction physicalmodels used in TPM. The RI map reconstruction algorithms and various regularization methods are discussed. Selected TPM applications for cellular imaging, particularly in hematology, are reviewed. Finally, we examine the limitations of current TPM systems, propose future solutions, and envision promising directions in biomedical research.
DepartmentMassachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Mechanical Engineering; Massachusetts Institute of Technology. Spectroscopy Laboratory
Journal of the Optical Society of America B
The Optical Society
Jin, Di, Renjie Zhou, Zahid Yaqoob, and Peter T. C. So. “Tomographic Phase Microscopy: Principles and Applications in Bioimaging [Invited].” Journal of the Optical Society of America B 34, no. 5 (April 4, 2017): B64.
Author's final manuscript