Diffraction optical tomography using a quantitative phase imaging unit
Author(s)Kim, Kyoohyun; Yaqoob, Zahid; Lee, Kyeoreh; Kang, Jeon Woong; Choi, Youngwoon; Hosseini, Poorya; So, Peter T. C.; Park, YongKeun; ... Show more Show less
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A simple and practical method to measure three-dimensional (3-D) refractive index (RI) distributions of biological cells is presented. A common-path self-reference interferometry consisting of a compact set of polarizers is attached to a conventional inverted microscope equipped with a beam scanning unit, which can precisely measure multiple 2-D holograms of a sample with high phase stability for various illumination angles, from which accurate 3-D optical diffraction tomograms of the sample can be reconstructed. 3-D RI tomograms of nonbiological samples such as polystyrene microspheres, as well as biological samples including human red blood cells and breast cancer cells, are presented.
DepartmentInstitute for Medical Engineering and Science; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Chemistry; Massachusetts Institute of Technology. Department of Mechanical Engineering; Massachusetts Institute of Technology. Spectroscopy Laboratory
The Optical Society
Kim, Kyoohyun, Zahid Yaqoob, KyeoReh Lee, Jeon Woong Kang, Youngwoon Choi, Poorya Hosseini, Peter T. C. So, and YongKeun Park. “Diffraction Optical Tomography Using a Quantitative Phase Imaging Unit.” Optics Letters 39, no. 24 (December 12, 2014): 6935.
Author's final manuscript