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Diffraction optical tomography using a quantitative phase imaging unit

Author(s)
Kim, Kyoohyun; Yaqoob, Zahid; Lee, Kyeoreh; Kang, Jeon Woong; Choi, Youngwoon; Hosseini, Poorya; So, Peter T. C.; Park, YongKeun; ... Show more Show less
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Abstract
A simple and practical method to measure three-dimensional (3-D) refractive index (RI) distributions of biological cells is presented. A common-path self-reference interferometry consisting of a compact set of polarizers is attached to a conventional inverted microscope equipped with a beam scanning unit, which can precisely measure multiple 2-D holograms of a sample with high phase stability for various illumination angles, from which accurate 3-D optical diffraction tomograms of the sample can be reconstructed. 3-D RI tomograms of nonbiological samples such as polystyrene microspheres, as well as biological samples including human red blood cells and breast cancer cells, are presented.
Date issued
2014-12
URI
http://hdl.handle.net/1721.1/120338
Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Chemistry; Massachusetts Institute of Technology. Department of Mechanical Engineering; Massachusetts Institute of Technology. Spectroscopy Laboratory
Journal
Optics Letters
Publisher
The Optical Society
Citation
Kim, Kyoohyun, Zahid Yaqoob, KyeoReh Lee, Jeon Woong Kang, Youngwoon Choi, Poorya Hosseini, Peter T. C. So, and YongKeun Park. “Diffraction Optical Tomography Using a Quantitative Phase Imaging Unit.” Optics Letters 39, no. 24 (December 12, 2014): 6935.
Version: Author's final manuscript
ISSN
0146-9592
1539-4794

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