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dc.contributor.authorSuguri, Takuya
dc.contributor.authorOlsen, Bradley D
dc.date.accessioned2019-03-05T20:34:00Z
dc.date.available2019-03-05T20:34:00Z
dc.date.issued2019-03
dc.date.submitted2018-08
dc.identifier.issn1759-9954
dc.identifier.issn1759-9962
dc.identifier.urihttp://hdl.handle.net/1721.1/120749
dc.description.abstractBioconjugates made of the model red fluorescent protein mCherry and synthetic polymer blocks show that topology, i.e. the BA, BA2, ABA and ABC chain structure of the block copolymers, where B represents the protein and A and C represent polymers, has a significant effect on ordering transitions and the type and size of nanostructures formed during microphase separation. ABA and ABC type block copolymers were synthesized by using two site-specific bioconjugation reactions: the thiol–ene reaction with a cysteine on mCherry and maleimide functionalized polymers, and the sortase A ligation reaction with an LPETG sequence at the C-terminus on mCherry and a triglycine functionalized polymer. The phase behaviors of mCherry–poly(N-isopropylacrylamide) (PNIPAM) and mCherry–(PNIPAM)2 show that the shapes of the phase diagrams are similar overall, but mCherry–(PNIPAM)2, i.e. BA2 type, yields a narrower domain spacing than mCherry–PNIPAM, i.e. BA type. PNIPAM–mCherry–PNIPAM (ABA type) shows only lamellar phases in the range of conditions under which ordered structures appear. PDMAPS–mCherry–PNIPAM (ABC type) shows an ordered structure across the widest range of conditions in the four bioconjugates and also the widest range of different nanodomain structures. The phase behavior of the ABC type implies that the repulsive interaction between two water-soluble coil polymers can be a key factor in enhancing the self-assembly of globular protein–polymer block copolymers.en_US
dc.description.sponsorshipDepartment of Energy Office of Basic Energy Sciences (Award DE-SC0007106)en_US
dc.language.isoen_US
dc.publisherRoyal Society of Chemistry, Theen_US
dc.relation.isversionofhttps://doi.org/10.1039/C8PY01228Hen_US
dc.rightsCreative Commons Attribution Noncommercial 3.0 unported licenseen_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc/3.0/en_US
dc.sourceRoyal Society of Chemistry (RSC)en_US
dc.titleTopology effects on protein–polymer block copolymer self-assemblyen_US
dc.typeArticleen_US
dc.identifier.citationSuguri, Takuya, and Bradley D. Olsen. “Topology Effects on Protein–polymer Block Copolymer Self-Assembly.” Polymer Chemistry (2019). doi:10.1039/c8py01228h.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.mitauthorSuguri, Takuya
dc.contributor.mitauthorOlsen, Bradley D
dc.relation.journalPolymer Chemistryen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsSuguri, Takuya; Olsen, Bradley D.en_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-7272-7140
mit.licensePUBLISHER_CCen_US


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