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dc.contributor.authorZhang, Fei
dc.contributor.authorQi, Xiaodong
dc.contributor.authorYan, Hao
dc.contributor.authorJun, Hyungmin
dc.contributor.authorShepherd, Tyson R
dc.contributor.authorRatanalert, Sakul
dc.contributor.authorBathe, Mark
dc.date.accessioned2019-03-07T14:12:07Z
dc.date.available2019-03-07T14:12:07Z
dc.date.issued2018-11
dc.date.submitted2018-08
dc.identifier.issn2375-2548
dc.identifier.urihttp://hdl.handle.net/1721.1/120776
dc.description.abstractScaffolded DNA origami offers the unique ability to organize molecules in nearly arbitrary spatial patterns at the nanometer scale, with wireframe designs further enabling complex 2D and 3D geometries with irregular boundaries and internal structures. The sequence design of the DNA staple strands needed to fold the long scaffold strand to the target geometry is typically performed manually, limiting the broad application of this materials design paradigm. Here, we present a fully autonomous procedure to design all DNA staple sequences needed to fold any free-form 2D scaffolded DNA origami wireframe object. Our algorithm uses wireframe edges consisting of two parallel DNA duplexes and enables the full autonomy of scaffold routing and staple sequence design with arbitrary network edge lengths and vertex angles. The application of our procedure to geometries with both regular and irregular external boundaries and variable internal structures demonstrates its broad utility for nanoscale materials science and nanotechnology.en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (Grant CCF-1564025)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (Grant CMMI-1334109)en_US
dc.description.sponsorshipOffice of Naval Research (Grant N000141210621)en_US
dc.publisherAmerican Association for the Advancement of Science (AAAS)en_US
dc.relation.isversionofhttp://dx.doi.org/10.1126/sciadv.aav0655en_US
dc.rightsCreative Commons Attribution NonCommercial License 4.0en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/en_US
dc.sourceScience Advancesen_US
dc.titleAutonomously designed free-form 2D DNA origamien_US
dc.typeArticleen_US
dc.identifier.citationJun, Hyungmin et al. “Autonomously Designed Free-Form 2D DNA Origami.” Science Advances 5, 1 (January 2019): eaav0655 © 2019 The Authorsen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.mitauthorJun, Hyungmin
dc.contributor.mitauthorShepherd, Tyson R
dc.contributor.mitauthorRatanalert, Sakul
dc.contributor.mitauthorBathe, Mark
dc.relation.journalScience Advancesen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2019-02-15T14:29:20Z
dspace.orderedauthorsJun, Hyungmin; Zhang, Fei; Shepherd, Tyson; Ratanalert, Sakul; Qi, Xiaodong; Yan, Hao; Bathe, Marken_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-7108-1288
dc.identifier.orcidhttps://orcid.org/0000-0001-7122-1917
dc.identifier.orcidhttps://orcid.org/0000-0002-1766-807X
dc.identifier.orcidhttps://orcid.org/0000-0002-6199-6855
mit.licensePUBLISHER_CCen_US


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