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dc.contributor.authorDouam, Florian
dc.contributor.authorHrebikova, Gabriela
dc.contributor.authorFant, Bruno
dc.contributor.authorLeach, Robert
dc.contributor.authorParsons, Lance
dc.contributor.authorWang, Wei
dc.contributor.authorGaska, Jenna M.
dc.contributor.authorWiner, Benjamin Y.
dc.contributor.authorHeller, Brigitte
dc.contributor.authorPloss, Alexander
dc.contributor.authorZiegler, Carly
dc.contributor.authorShalek, Alexander K
dc.date.accessioned2019-03-07T15:34:44Z
dc.date.available2019-03-07T15:34:44Z
dc.date.issued2018-11
dc.identifier.issn2041-1723
dc.identifier.urihttp://hdl.handle.net/1721.1/120790
dc.description.abstractMice engrafted with components of a human immune system have become widely-used models for studying aspects of human immunity and disease. However, a defined methodology to objectively measure and compare the quality of the human immune response in different models is lacking. Here, by taking advantage of the highly immunogenic live-attenuated yellow fever virus vaccine YFV-17D, we provide an in-depth comparison of immune responses in human vaccinees, conventional humanized mice, and second generation humanized mice. We demonstrate that selective expansion of human myeloid and natural killer cells promotes transcriptomic responses akin to those of human vaccinees. These enhanced transcriptomic profiles correlate with the development of an antigen-specific cellular and humoral response to YFV-17D. Altogether, our approach provides a robust scoring of the quality of the human immune response in humanized mice and highlights a rational path towards developing better pre-clinical models for studying the human immune response and disease.en_US
dc.description.sponsorshipNational Institute of General Medical Sciences (U.S.) (Award T32GM007753)en_US
dc.description.sponsorshipSearle Scholars Programen_US
dc.description.sponsorshipArnold and Mabel Beckman Foundation (Young Investigator Program)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (1DP2OD020839)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (5U24AI118672)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (1U54CA217377)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (1R33CA202820)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (2U19AI089992)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (21R01HL134539)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (2RM1HG006193)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (2R01HL095791)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (P01AI039671)en_US
dc.description.sponsorshipBill & Melinda Gates Foundation (OPP1139972)en_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/s41467-018-07478-2en_US
dc.rightsCreative Commons Attribution 4.0 International licenseen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.sourceNatureen_US
dc.titleSelective expansion of myeloid and NK cells in humanized mice yields human-like vaccine responsesen_US
dc.typeArticleen_US
dc.identifier.citationDouam, Florian, Carly G. K. Ziegler, Gabriela Hrebikova, Bruno Fant, Robert Leach, Lance Parsons, Wei Wang, et al. “Selective Expansion of Myeloid and NK Cells in Humanized Mice Yields Human-Like Vaccine Responses.” Nature Communications 9, no. 1 (November 28, 2018). © 2018 The Authorsen_US
dc.contributor.departmentMassachusetts Institute of Technology. Institute for Medical Engineering & Scienceen_US
dc.contributor.departmentHarvard University--MIT Division of Health Sciences and Technologyen_US
dc.contributor.departmentRagon Institute of MGH, MIT and Harvarden_US
dc.contributor.departmentKoch Institute for Integrative Cancer Research at MITen_US
dc.contributor.mitauthorZiegler, Carly
dc.contributor.mitauthorShalek, Alexander K
dc.relation.journalNature Communicationsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2019-03-04T14:52:02Z
dspace.orderedauthorsDouam, Florian; Ziegler, Carly G. K.; Hrebikova, Gabriela; Fant, Bruno; Leach, Robert; Parsons, Lance; Wang, Wei; Gaska, Jenna M.; Winer, Benjamin Y.; Heller, Brigitte; Shalek, Alex K.; Ploss, Alexanderen_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-8279-7150
dc.identifier.orcidhttps://orcid.org/0000-0001-5670-8778
mit.licensePUBLISHER_CCen_US


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