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dc.contributor.authorHan, Yu
dc.contributor.authorGoldberg, Jacob Michael
dc.contributor.authorLippard, Stephen J.
dc.contributor.authorPalmer, Amy E.
dc.date.accessioned2019-06-24T20:43:13Z
dc.date.available2019-06-24T20:43:13Z
dc.date.issued2018-09
dc.date.submitted2018-02
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/1721.1/121402
dc.description.abstractSmall-molecule fluorescent probes are powerful and ubiquitous tools for measuring the concentration and distribution of analytes in living cells. However, accurate characterization of these analytes requires rigorous evaluation of cell-to-cell heterogeneity in fluorescence intensities and intracellular distribution of probes. In this study, we perform a parallel and systematic comparison of two small-molecule fluorescent vesicular Zn²⁺ probes, FluoZin-3 AM and SpiroZin2, to evaluate each probe for measurement of vesicular Zn²⁺ pools. Our results reveal that SpiroZin2 is a specific lysosomal vesicular Zn²⁺ probe and affords uniform measurement of resting Zn²⁺ levels at the single cell level with proper calibration. In contrast, FluoZin-3 AM produces highly variable fluorescence intensities and non-specifically localizes in the cytosol and multiple vesicular compartments. We further applied SpiroZin2 to lactating mouse mammary epithelial cells and detected a transient increase of lysosomal free Zn²⁺ at 24-hour after lactation hormone treatment, which implies that lysosomes play a role in the regulation of Zn²⁺ homeostasis during lactation. This study demonstrates the need for critical characterization of small-molecule fluorescent probes to define the concentration and localization of analytes in different cell populations, and reveals SpiroZin2 to be capable of reporting diverse perturbations to lysosomal Zn²⁺.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant GM065519)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant F32 GM-109516)en_US
dc.publisherSpringer Natureen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/S41598-018-33102-Wen_US
dc.rightsCreative Commons Attribution 4.0 International licenseen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.sourceScientific Reportsen_US
dc.titleSuperiority of SpiroZin2 Versus FluoZin-3 for monitoring vesicular Zn²⁺ allows tracking of lysosomal Zn²⁺ poolsen_US
dc.typeArticleen_US
dc.identifier.citationHan, Yu, Jacob M. Goldberg, Stephen J. Lippard, and Amy E. Palmer. “Superiority of SpiroZin2 Versus FluoZin-3 for monitoring vesicular Zn²⁺ allows tracking of lysosomal Zn²⁺ pools.” Scientific Reports 8, 1 (October 2018): 15034 © 2018 The Author(s)en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemistryen_US
dc.relation.journalScientific Reportsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2019-03-25T15:33:07Z
dspace.orderedauthorsHan, Yu; Goldberg, Jacob M.; Lippard, Stephen J.; Palmer, Amy E.en_US
dspace.embargo.termsNen_US
dspace.date.submission2019-04-04T11:20:31Z
mit.journal.volume8en_US
mit.journal.issue1en_US
mit.licensePUBLISHER_CCen_US


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