dc.contributor.author | Han, Yu | |
dc.contributor.author | Goldberg, Jacob Michael | |
dc.contributor.author | Lippard, Stephen J. | |
dc.contributor.author | Palmer, Amy E. | |
dc.date.accessioned | 2019-06-24T20:43:13Z | |
dc.date.available | 2019-06-24T20:43:13Z | |
dc.date.issued | 2018-09 | |
dc.date.submitted | 2018-02 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://hdl.handle.net/1721.1/121402 | |
dc.description.abstract | Small-molecule fluorescent probes are powerful and ubiquitous tools for measuring the concentration and distribution of analytes in living cells. However, accurate characterization of these analytes requires rigorous evaluation of cell-to-cell heterogeneity in fluorescence intensities and intracellular distribution of probes. In this study, we perform a parallel and systematic comparison of two small-molecule fluorescent vesicular Zn²⁺ probes, FluoZin-3 AM and SpiroZin2, to evaluate each probe for measurement of vesicular Zn²⁺ pools. Our results reveal that SpiroZin2 is a specific lysosomal vesicular Zn²⁺ probe and affords uniform measurement of resting Zn²⁺ levels at the single cell level with proper calibration. In contrast, FluoZin-3 AM produces highly variable fluorescence intensities and non-specifically localizes in the cytosol and multiple vesicular compartments. We further applied SpiroZin2 to lactating mouse mammary epithelial cells and detected a transient increase of lysosomal free Zn²⁺ at 24-hour after lactation hormone treatment, which implies that lysosomes play a role in the regulation of Zn²⁺ homeostasis during lactation. This study demonstrates the need for critical characterization of small-molecule fluorescent probes to define the concentration and localization of analytes in different cell populations, and reveals SpiroZin2 to be capable of reporting diverse perturbations to lysosomal Zn²⁺. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant GM065519) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant F32 GM-109516) | en_US |
dc.publisher | Springer Nature | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1038/S41598-018-33102-W | en_US |
dc.rights | Creative Commons Attribution 4.0 International license | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_US |
dc.source | Scientific Reports | en_US |
dc.title | Superiority of SpiroZin2 Versus FluoZin-3 for monitoring vesicular Zn²⁺ allows tracking of lysosomal Zn²⁺ pools | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Han, Yu, Jacob M. Goldberg, Stephen J. Lippard, and Amy E. Palmer. “Superiority of SpiroZin2 Versus FluoZin-3 for monitoring vesicular Zn²⁺ allows tracking of lysosomal Zn²⁺ pools.” Scientific Reports 8, 1 (October 2018): 15034 © 2018 The Author(s) | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Chemistry | en_US |
dc.relation.journal | Scientific Reports | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2019-03-25T15:33:07Z | |
dspace.orderedauthors | Han, Yu; Goldberg, Jacob M.; Lippard, Stephen J.; Palmer, Amy E. | en_US |
dspace.embargo.terms | N | en_US |
dspace.date.submission | 2019-04-04T11:20:31Z | |
mit.journal.volume | 8 | en_US |
mit.journal.issue | 1 | en_US |
mit.license | PUBLISHER_CC | en_US |