Imaging cellular ultrastructures using expansion microscopy (U-ExM)
Author(s)Gambarotto, Davide; Zwettler, Fabian U.; Le Guennec, Maeva; Schmidt-Cernohorska, Marketa; Fortun, Denis; Heine, Jörn; Schloetel, Jan-Gero; Reuss, Matthias; Unser, Michael; Boyden, E; Sauer, Markus; Hamel, Virginie; Guichard, Paul; ... Show more Show less
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Determining the structure and composition of macromolecular assemblies is a major challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an extension of expansion microscopy that allows the visualization of preserved ultrastructures by optical microscopy. This method allows for near-native expansion of diverse structures in vitro and in cells; when combined with super-resolution microscopy, it unveiled details of ultrastructural organization, such as centriolar chirality, that could otherwise be observed only by electron microscopy.
DepartmentMassachusetts Institute of Technology. Department of Biology
Gambarotto, Davide et al. "Imaging cellular ultrastructures using expansion microscopy (U-ExM)." Nature Methods 16, 1 (December 2018): 71-74 © 2018 The Author(s)
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