CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion
Author(s)Yin, Hao; Wu, Qiongqiong; Anderson, Daniel Griffith; Weng, Zhiping
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CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of β-catenin exon 3, which encodes an autoinhibitory domain, induces partial skipping of the in-frame exon and nuclear accumulation of β-catenin. A single sgRNA can induce small insertions or deletions that partially alter splicing or unexpected larger deletions that remove exons. Exon skipping adds to the unexpected outcomes that must be accounted for, and perhaps taken advantage of, in CRISPR experiments.
DepartmentHarvard University--MIT Division of Health Sciences and Technology; Koch Institute for Integrative Cancer Research at MIT
Mou, Haiwei, Jordan L. Smith, Lingtao Peng, Hao Yin, Jill Moore, Xiao-Ou Zhang, Chun-Qing Song,Ankur Sheel, Qiongqiong Wu, Deniz M. Ozata, Yingxiang Li, Daniel G. Anderson, Charles P. Emerson, Erik J. Sontheimer, Melissa J. Moore,*, Zhiping Weng and Wen Xue. "CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion." Genome Biology, 18, no. 1 (June 2017): article no. 108 © 2017 The Author(s)
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