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dc.contributor.authorUrrejola, Catalina
dc.contributor.authorAlcorta, Jaime
dc.contributor.authorSalas, Loreto
dc.contributor.authorVásquez, Mónica
dc.contributor.authorPolz, Martin F
dc.contributor.authorVicuña, Rafael
dc.contributor.authorDíez, Beatriz
dc.date.accessioned2020-01-22T21:33:31Z
dc.date.available2020-01-22T21:33:31Z
dc.date.issued2019-05
dc.date.submitted2018-05
dc.identifier.issn1664-302X
dc.identifier.urihttps://hdl.handle.net/1721.1/123543
dc.description.abstractFor tolerating extreme desiccation, cyanobacteria are known to produce both compatible solutes at intracellular level and a copious amount of exopolysaccharides as a protective coat. However, these molecules make cyanobacterial cells refractory to a broad spectrum of cell disruption methods, hindering genome sequencing, and molecular studies. In fact, few genomes are already available from cyanobacteria from extremely desiccated environments such as deserts. In this work, we report the 5.4 Mbp draft genome (with 100% of completeness in 105 contigs) of Gloeocapsopsis sp. UTEX B3054 (subsection I; Order Chroococcales), a cultivable sugar-rich and hardly breakable hypolithic cyanobacterium from the Atacama Desert. Our in silico analyses focused on genomic features related to sugar-biosynthesis and adaptation to dryness. Among other findings, screening of Gloeocapsopsis genome revealed a unique genetic potential related to the biosynthesis and regulation of compatible solutes and polysaccharides. For instance, our findings showed for the first time a novel genomic arrangement exclusive of Chroococcaceae cyanobacteria associated with the recycling of trehalose, a compatible solute involved in desiccation tolerance. Additionally, we performed a comparative genome survey and analyses to entirely predict the highly diverse pool of glycosyltransferases enzymes, key players in polysaccharide biosynthesis and the formation of a protective coat to dryness. We expect that this work will set the fundamental genomic framework for further research on microbial tolerance to desiccation and to a wide range of other extreme environmental conditions. The study of microorganisms like Gloeocapsopsis sp. UTEX B3054 will contribute to expand our limited understanding regarding water optimization and molecular mechanisms allowing extremophiles to thrive in xeric environments such as the Atacama Desert.en_US
dc.language.isoen
dc.publisherFrontiers Mediaen_US
dc.relation.isversionofhttp://dx.doi.org/10.3389/fmicb.2019.00950en_US
dc.rightsCreative Commons Attribution 4.0 International licenseen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.sourceFrontiersen_US
dc.titleGenomic Features for Desiccation Tolerance and Sugar Biosynthesis in the Extremophile Gloeocapsopsis sp. UTEX B3054en_US
dc.typeArticleen_US
dc.identifier.citationUrrejola, Catalina et al. "Genomic Features for Desiccation Tolerance and Sugar Biosynthesis in the Extremophile Gloeocapsopsis sp. UTEX B3054." Frontiers in Microbiology 10 (May 2019): 950 © 2019 Frontiers Mediaen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Civil and Environmental Engineeringen_US
dc.relation.journalFrontiers in Microbiologyen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2020-01-22T13:01:04Z
dspace.date.submission2020-01-22T13:01:08Z
mit.journal.volume10en_US
mit.licensePUBLISHER_CC


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