dc.contributor.author | Gutzman, Jennifer H. | |
dc.contributor.author | Graeden, Ellie | |
dc.contributor.author | Brachmann, Isabel | |
dc.contributor.author | Sive, Hazel | |
dc.date.accessioned | 2020-04-15T17:11:53Z | |
dc.date.available | 2020-04-15T17:11:53Z | |
dc.date.issued | 2018-10-10 | |
dc.identifier.issn | 2046-6390 | |
dc.identifier.uri | https://hdl.handle.net/1721.1/124660 | |
dc.description.abstract | Basal constriction occurs at the zebrafish midbrain-hindbrain boundary constriction (MHBC) and is likely a widespread morphogenetic mechanism. 3D reconstruction demonstrates that MHBC cells are wedge-shaped, and initially constrict basally, with subsequent apical expansion. wnt5b is expressed in the MHB and is required for basal constriction. Consistent with a requirement for this pathway, expression of dominant negative Gsk3β overcomes wnt5b knockdown. Immunostaining identifies focal adhesion kinase (Fak) as active in the MHB region, and knockdown demonstrates Fak is a regulator of basal constriction. Tissue specific knockdown further indicates that Fak functions cell autonomously within the MHBC. Fak acts downstream of wnt5b, suggesting that Wnt5b signals locally as an early step in basal constriction and acts together with more widespread Fak activation. This study delineates signaling pathways that regulate basal constriction during brain morphogenesis. | en_US |
dc.description.sponsorship | National Science Foundation (U.S.). Graduate Research Fellowship (Grant 1122374) | en_US |
dc.description.sponsorship | National Science Foundation (U.S.) (Grant 1258087) | en_US |
dc.language.iso | en | |
dc.publisher | The Company of Biologists | en_US |
dc.relation.isversionof | 10.1242/bio.034520 | en_US |
dc.rights | Creative Commons Attribution 4.0 International license | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_US |
dc.source | The Company of Biologists | en_US |
dc.title | Basal constriction during midbrain–hindbrain boundary morphogenesis is mediated by Wnt5b and focal adhesion kinase | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Gutzman, Jennifer H., Ellie Graeden, Isabel Brachmann, Sayumi Yamazoe, James K. Chen, and Hazel Sive. "Basal constriction during midbrain–hindbrain boundary morphogenesis is mediated by Wnt5b and focal adhesion kinase." Biology open 7 (2018): bio034520 © 2018 The Author(s) | en_US |
dc.contributor.department | Whitehead Institute for Biomedical Research | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.relation.journal | Biology open | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2020-01-30T17:52:14Z | |
dspace.date.submission | 2020-01-30T17:52:17Z | |
mit.journal.volume | 7 | en_US |
mit.journal.issue | 11 | en_US |
mit.license | PUBLISHER_CC | |
mit.metadata.status | Complete | |