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dc.contributor.authorKersten, Roland D.
dc.contributor.authorWeng, Jing-Ke
dc.date.accessioned2020-04-15T17:12:54Z
dc.date.available2020-04-15T17:12:54Z
dc.date.issued2018-10-29
dc.identifier.issn0027-8424
dc.identifier.issn1091-6490
dc.identifier.urihttps://hdl.handle.net/1721.1/124661
dc.description.abstractThe plant kingdom contains vastly untapped natural product chemistry, which has been traditionally explored through the activity-guided approach. Here, we describe a gene-guided approach to discover and engineer a class of plant ribosomal peptides, the branched cyclic lyciumins. Initially isolated from the Chinese wolfberry Lycium barbarum, lyciumins are protease-inhibiting peptides featuring an N-terminal pyroglutamate and a macrocyclic bond between a tryptophan-indole nitrogen and a glycine α-carbon. We report the identification of a lyciumin precursor gene from L. barbarum, which encodes a BURP domain and repetitive lyciumin precursor peptide motifs. Genome mining enabled by this initial finding revealed rich lyciumin genotypes and chemotypes widespread in flowering plants. We establish a biosynthetic framework of lyciumins and demonstrate the feasibility of producing diverse natural and unnatural lyciumins in transgenic tobacco. With rapidly expanding plant genome resources, our approach will complement bioactivity-guided approaches to unlock and engineer hidden plant peptide chemistry for pharmaceutical and agrochemical applications.en_US
dc.language.isoen
dc.publisherProceedings of the National Academy of Sciencesen_US
dc.relation.isversionof10.1073/pnas.1813993115en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePNASen_US
dc.subjectMultidisciplinaryen_US
dc.titleGene-guided discovery and engineering of branched cyclic peptides in plantsen_US
dc.typeArticleen_US
dc.identifier.citationKersten, Roland D. and Jing-Ke Weng. "Gene-guided discovery and engineering of branched cyclic peptides in plants." Proceedings of the National Academy of Sciences of the United States of America 115 (2018): E10961-E10969 © 2019 The Author(s)en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentWhitehead Institute for Biomedical Researchen_US
dc.relation.journalProceedings of the National Academy of Sciences of the United States of Americaen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2020-02-03T17:33:50Z
dspace.date.submission2020-02-03T17:33:52Z
mit.journal.volume115en_US
mit.journal.issue46en_US
mit.licensePUBLISHER_POLICY
mit.metadata.statusComplete


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