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dc.contributor.authorKory, Nora
dc.contributor.authorWyant, Gregory A.
dc.contributor.authorPrakash, Gyan
dc.contributor.authorDe Bos, Jelmi uit
dc.contributor.authorPacold, Michael E.
dc.contributor.authorChan, Sze Ham
dc.contributor.authorLewis, Caroline A.
dc.contributor.authorWang, Tim
dc.contributor.authorKeys, Heather R.
dc.contributor.authorSabatini, David M.
dc.date.accessioned2020-04-16T15:35:21Z
dc.date.available2020-04-16T15:35:21Z
dc.date.issued2018-11
dc.identifier.issn0193-4511
dc.identifier.urihttps://hdl.handle.net/1721.1/124696
dc.description.abstractOne-carbon metabolism generates the one-carbon units required to synthesize many critical metabolites, including nucleotides. The pathway has cytosolic and mitochondrial branches, and a key step is the entry, through an unknown mechanism, of serine into mitochondria, where it is converted into glycine and formate. In a CRISPR-based genetic screen in human cells for genes of the mitochondrial pathway, we found sideroflexin 1 (SFXN1), a multipass inner mitochondrial membrane protein of unclear function. Like cells missing mitochondrial components of one-carbon metabolism, those null for SFXN1 are defective in glycine and purine synthesis. Cells lacking SFXN1 and one of its four homologs, SFXN3, have more severe defects, including being auxotrophic for glycine. Purified SFXN1 transports serine in vitro. Thus, SFXN1 functions as a mitochondrial serine transporter in one-carbon metabolism.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant R01 CA103866)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant R01 CA129105)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant R37 AI47389)en_US
dc.description.sponsorshipUnited States. Department of Defense (Grant W81XWH-07–0448)en_US
dc.language.isoen
dc.publisherAmerican Association for the Advancement of Science (AAAS)en_US
dc.relation.isversionof10.1126/SCIENCE.AAT9528en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleSFXN1 is a mitochondrial serine transporter required for one-carbon metabolismen_US
dc.typeArticleen_US
dc.identifier.citationKory, Nora et al. "SFXN1 is a mitochondrial serine transporter required for one-carbon metabolism." Science 362 (2018): aat9528 © 2018 The Authorsen_US
dc.contributor.departmentWhitehead Institute for Biomedical Researchen_US
dc.contributor.departmentKoch Institute for Integrative Cancer Research at MITen_US
dc.relation.journalScienceen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2020-01-30T14:19:53Z
dspace.date.submission2020-01-30T14:19:54Z
mit.journal.volume362en_US
mit.licenseOPEN_ACCESS_POLICY
mit.metadata.statusComplete


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