Selective gene dependencies in MYCN-amplified neuroblastoma include the core transcriptional regulatory circuitry
Author(s)Abraham, Brian J.; Young, Richard A.
MetadataShow full item record
Childhood high-risk neuroblastomas with MYCN gene amplification are difficult to treat effectively 1 . This has focused attention on tumor-specific gene dependencies that underlie tumorigenesis and thus provide valuable targets for the development of novel therapeutics. Using unbiased genome-scale CRISPR–Cas9 approaches to detect genes involved in tumor cell growth and survival 2–6 , we identified 147 candidate gene dependencies selective for MYCN-amplified neuroblastoma cell lines, compared to over 300 other human cancer cell lines. We then used genome-wide chromatin-immunoprecipitation coupled to high-throughput sequencing analysis to demonstrate that a small number of essential transcription factors—MYCN, HAND2, ISL1, PHOX2B, GATA3, and TBX2—are members of the transcriptional core regulatory circuitry (CRC) that maintains cell state in MYCN-amplified neuroblastoma. To disable the CRC, we tested a combination of BRD4 and CDK7 inhibitors, which act synergistically, in vitro and in vivo, with rapid downregulation of CRC transcription factor gene expression. This study defines a set of critical dependency genes in MYCN-amplified neuroblastoma that are essential for cell state and survival in this tumor.
DepartmentWhitehead Institute for Biomedical Research; Massachusetts Institute of Technology. Department of Biology
Springer Science and Business Media LLC
Durbin, Adam D. et al. “Selective gene dependencies in MYCN-amplified neuroblastoma include the core transcriptional regulatory circuitry.” Nature genetics 50 (2018): 1240-1246 © 2018 The Author(s)
Author's final manuscript