dc.contributor.author | Regev, Aviv | |
dc.date.accessioned | 2020-05-14T18:37:07Z | |
dc.date.available | 2020-05-14T18:37:07Z | |
dc.date.issued | 2019-02 | |
dc.identifier.issn | 0092-8674 | |
dc.identifier.uri | https://hdl.handle.net/1721.1/125240 | |
dc.description.abstract | High-acuity vision in primates, including humans, is mediated by a small central retinal region called the fovea. As more accessible organisms lack a fovea, its specialized function and its dysfunction in ocular diseases remain poorly understood. We used 165,000 single-cell RNA-seq profiles to generate comprehensive cellular taxonomies of macaque fovea and peripheral retina. More than 80% of >60 cell types match between the two regions but exhibit substantial differences in proportions and gene expression, some of which we relate to functional differences. Comparison of macaque retinal types with those of mice reveals that interneuron types are tightly conserved. In contrast, projection neuron types and programs diverge, despite exhibiting conserved transcription factor codes. Key macaque types are conserved in humans, allowing mapping of cell-type and region-specific expression of >190 genes associated with 7 human retinal diseases. Our work provides a framework for comparative single-cell analysis across tissue regions and species. Single-cell-based analysis provides a comprehensive molecular and cellular taxonomy of the primate retina. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant MH105960) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant EY025840) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant EY028633) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant EY025555) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant EY028625) | en_US |
dc.description.sponsorship | BrightFocus Foundation (Grant M2014055) | en_US |
dc.language.iso | en | |
dc.publisher | Elsevier BV | en_US |
dc.relation.isversionof | 10.1016/J.CELL.2019.01.004 | en_US |
dc.rights | Creative Commons Attribution-NonCommercial-NoDerivs License | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en_US |
dc.source | PMC | en_US |
dc.title | Molecular Classification and Comparative Taxonomics of Foveal and Peripheral Cells in Primate Retina | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Peng, Yi-Rong et al. “Molecular Classification and Comparative Taxonomics of Foveal and Peripheral Cells in Primate Retina.” Cell 176 (2019): 1222-1237.e22 © 2019 The Author(s) | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.department | Koch Institute for Integrative Cancer Research at MIT | en_US |
dc.relation.journal | Cell | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2020-03-04T13:55:23Z | |
dspace.date.submission | 2020-03-04T13:55:25Z | |
mit.journal.volume | 176 | en_US |
mit.journal.issue | 5 | en_US |
mit.license | PUBLISHER_CC | |
mit.metadata.status | Complete | |