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dc.contributor.authorMorgan, Jeffrey T. (Jeffrey Thomas)
dc.contributor.authorFink, Gerald R.
dc.contributor.authorBartel, David
dc.date.accessioned2020-05-15T12:49:32Z
dc.date.available2020-05-15T12:49:32Z
dc.date.issued2019-01
dc.date.submitted2017-12
dc.identifier.issn0028-0836
dc.identifier.issn1476-4687
dc.identifier.urihttps://hdl.handle.net/1721.1/125253
dc.description.abstractSpliceosomal introns are ubiquitous non-coding RNAs that are typically destined for rapid debranching and degradation. Here we describe 34 excised introns in Saccharomyces cerevisiae that—despite being rapidly degraded in log-phase growth—accumulate as linear RNAs under either saturated-growth conditions or other stresses that cause prolonged inhibition of TORC1, which is a key integrator of growth signalling. Introns that become stabilized remain associated with components of the spliceosome and differ from other spliceosomal introns in having a short distance between their lariat branch point and 3′ splice site, which is necessary and sufficient for their stabilization. Deletion of these unusual introns is disadvantageous in saturated conditions and causes aberrantly high growth rates in yeast that are chronically challenged with the TORC1 inhibitor rapamycin. The reintroduction of native or engineered stable introns suppresses this aberrant rapamycin response. Thus, excised introns function within the TOR growth-signalling network of S. cerevisiae and, more generally, excised spliceosomal introns can have biological functions. Keywords: Non-coding RNAs; RNA; TOR signalling; Transcriptomicsen_US
dc.description.sponsorshipNIH (Grant GM035010)en_US
dc.description.sponsorshipNIH (Grant GM118135)en_US
dc.language.isoen
dc.publisherSpringer Natureen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/s41586-018-0828-1en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePMCen_US
dc.titleExcised linear introns regulate growth in yeasten_US
dc.typeArticleen_US
dc.identifier.citationMorgan1, Jeffrey T., Fink, Gerald R. and Bartel, David P. "Excised linear introns regulate growth in yeast." Nature 565, 7741 (January 2019): 606–611 © 2019, Springer Nature Limited.en_US
dc.contributor.departmentWhitehead Institute for Biomedical Researchen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.relation.journalNatureen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2019-11-26T18:15:58Z
dspace.date.submission2019-11-26T18:16:00Z
mit.journal.volume565en_US
mit.journal.issue7741en_US


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