dc.contributor.author | Nagarajan, Maxwell Benjamin | |
dc.contributor.author | Tentori, Augusto M | |
dc.contributor.author | Zhang, Wen Cai | |
dc.contributor.author | Slack, Frank J. | |
dc.contributor.author | Doyle, Patrick S | |
dc.date.accessioned | 2020-05-15T15:15:03Z | |
dc.date.available | 2020-05-15T15:15:03Z | |
dc.date.issued | 2018-08 | |
dc.date.submitted | 2018-05 | |
dc.identifier.issn | 0003-2700 | |
dc.identifier.issn | 1520-6882 | |
dc.identifier.uri | https://hdl.handle.net/1721.1/125269 | |
dc.description.abstract | MicroRNAs (miRNA) are short, noncoding RNAs that have been implicated in many diseases, including cancers. Because miRNAs are dysregulated in disease, miRNAs show promise as highly stable biomarkers. Formalin-fixed, paraffin-embedded (FFPE) tissue is a valuable sample type to assay for biomolecules because it is a convenient storage method and is often used by pathologists for histological staining. However, extracting biomolecules from FFPE tissue is challenging because of the presence of cellular and extracellular proteins, formaldehyde cross-links, and paraffin. Moreover, most protocols to measure miRNA in FFPE tissue are time-consuming and laborious. Here, we report a simple protocol to directly measure miRNA from formalin-fixed cells, FFPE tissue sections after paraffin is removed, and FFPE tissue sections using encoded hydrogel microparticles fabricated using stop flow lithography. Measurements by these particles show agreement between formalin-fixed cells and fresh cells, and measurement of FFPE tissue with paraffin is 10% less than FFPE tissue when paraffin is removed before the assay. When normal and tumor FFPE tissue are compared using this microparticle assay, we observe differential miRNA signal for oncogenic miRNAs and tumor suppressing miRNAs. This approach reduces assay times, reduces the use of hazardous chemicals to remove paraffin, and provides a sensitive, quantitative, and multiplexed measurement of miRNA in FFPE tissue. | en_US |
dc.description.sponsorship | NIH-NIBIB (Grant 5R21EB024101-02) | en_US |
dc.language.iso | en | |
dc.publisher | American Chemical Society (ACS) | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1021/acs.analchem.8b02010 | en_US |
dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
dc.source | PMC | en_US |
dc.title | Nonfouling, Encoded Hydrogel Microparticles for Multiplex MicroRNA Profiling Directly from Formalin-Fixed, Paraffin-Embedded Tissue | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Nagarajan, Maxwell Benjamin et al. "Nonfouling, Encoded Hydrogel Microparticles for Multiplex MicroRNA Profiling Directly from Formalin-Fixed, Paraffin-Embedded Tissue." Analytical Chemistry 90, 17 (August 2018): 10279-10285 © 2018 American Chemical Society | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Chemical Engineering | en_US |
dc.relation.journal | Analytical Chemistry | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2019-10-02T13:02:58Z | |
dspace.date.submission | 2019-10-02T13:02:59Z | |
mit.journal.volume | 90 | en_US |
mit.journal.issue | 17 | en_US |
mit.metadata.status | Complete | |