| dc.contributor.author | Hsu, Jonathan Y. | |
| dc.contributor.author | Fulco, Charles P. | |
| dc.contributor.author | Farouni, Rick | |
| dc.contributor.author | Clement, Kendell | |
| dc.contributor.author | Engreitz, Jesse Michael | |
| dc.contributor.author | Lander, Eric Steven | |
| dc.contributor.author | Pinello, Luca | |
| dc.date.accessioned | 2020-05-21T19:12:31Z | |
| dc.date.available | 2020-05-21T19:12:31Z | |
| dc.date.issued | 2018-12 | |
| dc.identifier.issn | 1548-7091 | |
| dc.identifier.uri | https://hdl.handle.net/1721.1/125387 | |
| dc.description.abstract | Tiling screens that use CRISPR-Cas technologies provide a powerful approach for the mapping of regulatory elements to phenotypes of interest1–6. Here we present CRISPR screening uncharacterized region function (CRISPR-SURF), a deconvolution framework that can be used to identify functional regulatory regions in the genome from data generated by CRISPR-Cas nuclease, CRISPR interference (CRISPRi), or CRISPR activation (CRISPRa) tiling screens. CRISPR-SURF can be run as a stand-alone command line utility (https://github.com/pinellolab/CRISPR-SURF) or as a web application (http://crisprsurf.pinellolab.org/) | en_US |
| dc.description.sponsorship | National Human Genome Research Institute (U.S.) (Career Development Award R00 HG008399) | en_US |
| dc.description.sponsorship | National Human Genome Research Institute (U.S.). Centers of Excellence in Genomic Science (Grant RM1HG009490) | en_US |
| dc.description.sponsorship | National Institutes of Health (U.S.) (Grant R35 GM118158) | en_US |
| dc.description.sponsorship | National Institutes of Health (U.S.) (Grant RM1 HG009490) | en_US |
| dc.description.sponsorship | National Human Genome Research Institute (U.S.) (Grant 1K99HG009917-01) | en_US |
| dc.language.iso | en | |
| dc.publisher | Springer Science and Business Media LLC | en_US |
| dc.relation.isversionof | 10.1038/S41592-018-0225-6 | en_US |
| dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
| dc.source | PMC | en_US |
| dc.title | CRISPR-SURF: discovering regulatory elements by deconvolution of CRISPR tiling screen data | en_US |
| dc.type | Article | en_US |
| dc.identifier.citation | Hsu, Jonathan Y. et al. “CRISPR-SURF: discovering regulatory elements by deconvolution of CRISPR tiling screen data.” Nature methods 15 (2018): 992-993 © 2018 The Author(s) | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
| dc.relation.journal | Nature methods | en_US |
| dc.eprint.version | Author's final manuscript | en_US |
| dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
| eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
| dc.date.updated | 2020-01-22T17:53:53Z | |
| dspace.date.submission | 2020-01-22T17:53:55Z | |
| mit.journal.volume | 15 | en_US |
| mit.journal.issue | 12 | en_US |
| mit.metadata.status | Complete | |
