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dc.contributor.authorXue, Yi
dc.contributor.authorBerry, Kalen Paul
dc.contributor.authorBoivin, Josiah R.
dc.contributor.authorRowlands, Christopher
dc.contributor.authorTakiguchi, Yu
dc.contributor.authorNedivi, Elly
dc.contributor.authorSo, Peter T. C.
dc.date.accessioned2020-05-26T19:46:18Z
dc.date.available2020-05-26T19:46:18Z
dc.date.issued2019-01
dc.date.submitted2018-10
dc.identifier.issn2334-2536
dc.identifier.urihttps://hdl.handle.net/1721.1/125461
dc.description.abstractSimultaneous, high-resolution imaging across a large number of synaptic and dendritic sites is critical for understanding how neurons receive and integrate signals. Yet, functional imaging that targets a large number of submicrometer-sized synaptic and dendritic locations poses significant technical challenges. We demonstrate a new parallelized approach to address such questions, increasing the signal-to-noise ratio by an order of magnitude compared to previous approaches. This selective access multifocal multiphoton microscopy uses a spatial light modulator to generate multifocal excitation in three dimensions (3D) and a Gaussian–Laguerre phase plate to simultaneously detect fluorescence from these spots throughout the volume. We test the performance of this system by simultaneously recording Ca 2 dynamics from cultured neurons at 98–118 locations distributed throughout a 3D volume. This is the first demonstration of 3D imaging in a “single shot” and permits synchronized monitoring of signal propagation across multiple different dendrites.en_US
dc.description.sponsorshipNational Institutes of Health (NIH) (Grant 1-R01-HL121386-01A1)en_US
dc.description.sponsorshipNational Institutes of Health (NIH) (Grant 1R21NS105070-01)en_US
dc.description.sponsorshipNational Institutes of Health (NIH) (Grant 1U01CA202177-01)en_US
dc.description.sponsorshipNational Institutes of Health (NIH) (Grant 1-U01-NS090438-01)en_US
dc.description.sponsorshipNational Institutes of Health (NIH) (Grant 5-P41-EB015871-27)en_US
dc.description.sponsorshipNational Institutes of Health (NIH) (Grant 5R21NS091982-02)en_US
dc.description.sponsorshipNational Institutes of Health (NIH) (Grant F32 MH115441)en_US
dc.language.isoen
dc.publisherThe Optical Societyen_US
dc.relation.isversionofhttps://dx.doi.org/10.1364/optica.6.000076en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceOSA Publishingen_US
dc.titleScanless volumetric imaging by selective access multifocal multiphoton microscopyen_US
dc.typeArticleen_US
dc.identifier.citationXue, Yi et al. "Scanless volumetric imaging by selective access multifocal multiphoton microscopy." Optica 6, 1 (January 2019): 76-83 © 2019 Optical Society of America.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Laser Biomedical Research Centeren_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentPicower Institute for Learning and Memoryen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Brain and Cognitive Sciencesen_US
dc.relation.journalOpticaen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2019-10-03T16:04:41Z
dspace.date.submission2019-10-03T16:04:44Z
mit.journal.volume6en_US
mit.journal.issue1en_US
mit.metadata.statusComplete


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