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dc.contributor.authorBecker, Jamie William
dc.contributor.authorHogle, Shane Lahman
dc.contributor.authorRosendo, Kali M
dc.contributor.authorChisholm, Sallie (Penny)
dc.date.accessioned2020-05-28T15:16:15Z
dc.date.available2020-05-28T15:16:15Z
dc.date.issued2019-02
dc.date.submitted2019-01
dc.identifier.issn1751-7362
dc.identifier.issn1751-7370
dc.identifier.urihttps://hdl.handle.net/1721.1/125545
dc.description.abstractProchlorococcus and SAR11 are among the smallest and most abundant organisms on Earth. With a combined global population of about 2.7 × 1028 cells, they numerically dominate bacterioplankton communities in oligotrophic ocean gyres and yet they have never been grown together in vitro. Here we describe co-cultures of Prochlorococcus and SAR11 isolates representing both high- and low-light adapted clades. We examined: (1) the influence of Prochlorococcus on the growth of SAR11 and vice-versa, (2) whether Prochlorococcus can meet specific nutrient requirements of SAR11, and (3) how co-culture dynamics vary when Prochlorococcus is grown with SAR11 compared with sympatric copiotrophic bacteria. SAR11 grew 15–70% faster in co-culture with Prochlorococcus, while the growth of the latter was unaffected. When Prochlorococcus populations entered stationary phase, this commensal relationship rapidly became amensal, as SAR11 abundances decreased dramatically. In parallel experiments with copiotrophic bacteria; however, the heterotrophic partner increased in abundance as Prochlorococcus densities leveled off. The presence of Prochlorococcus was able to meet SAR11’s central requirement for organic carbon, but not reduced sulfur. Prochlorococcus strain MIT9313, but not MED4, could meet the unique glycine requirement of SAR11, which could be due to the production and release of glycine betaine by MIT9313, as supported by comparative genomic evidence. Our findings also suggest, but do not confirm, that Prochlorococcus MIT9313 may compete with SAR11 for the uptake of 3-dimethylsulfoniopropionate (DMSP). To give our results an ecological context, we assessed the relative contribution of Prochlorococcus and SAR11 genome equivalents to those of identifiable bacteria and archaea in over 800 marine metagenomes. At many locations, more than half of the identifiable genome equivalents in the euphotic zone belonged to Prochlorococcus and SAR11 – highlighting the biogeochemical potential of these two groups.en_US
dc.description.sponsorshipNational Science Foundation (U.S.). (Grant DBI-0424599)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (Grant OCE-1153588)en_US
dc.description.sponsorshipSimons Foundation (Life Sciences Project Award ID 337262, SCOPE award ID 329108)en_US
dc.description.sponsorshipGordon and Betty Moore Foundation (Grant IDs GBMF495 and GBMF4511)en_US
dc.language.isoen
dc.publisherSpringer Science and Business Media LLCen_US
dc.relation.isversionofhttps://dx.doi.org/10.1038/S41396-019-0365-4en_US
dc.rightsCreative Commons Attribution 4.0 International licenseen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.sourceISME Journalen_US
dc.titleCo-culture and biogeography of Prochlorococcus and SAR11en_US
dc.typeArticleen_US
dc.identifier.citationBecker, Jamie W., et al. "Co-culture and biogeography of Prochlorococcus and SAR11." The ISME Journal (2019) 13:1506–1519 © 2019, The Author(s).en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Civil and Environmental Engineeringen_US
dc.relation.journalISME Journalen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2020-05-20T18:47:38Z
dspace.date.submission2020-05-20T18:47:40Z
mit.journal.volume13en_US
mit.journal.issue6en_US
mit.licensePUBLISHER_CC


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