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dc.contributor.authorLeventhal, Gabriel Etan
dc.contributor.authorBoix, Carles
dc.contributor.authorEnke, Tim Niklas
dc.contributor.authorCordero Sanchez, Otto X.
dc.date.accessioned2020-06-04T18:21:51Z
dc.date.available2020-06-04T18:21:51Z
dc.date.issued2018-09
dc.identifier.issn2058-5276
dc.identifier.urihttps://hdl.handle.net/1721.1/125676
dc.description.abstractMicrobial communities are often highly diverse in their composition, both at a coarse-grained taxonomic level, such as genus, and at a highly resolved level, such as strains, within species. This variability can be driven by either extrinsic factors such as temperature and or by intrinsic ones, for example demographic fluctuations or ecological interactions. The relative contributions of these factors and the taxonomic level at which they influence community composition remain poorly understood, in part because of the difficulty in identifying true community replicates assembled under the same environmental parameters. Here, we address this problem using an activated granular sludge reactor in which millimetre-scale biofilm granules represent true community replicates. Differences in composition are then expected to be driven primarily by biotic factors. Using 142 shotgun metagenomes of single biofilm granules we found that, at the commonly used genus-level resolution, community replicates varied much more in their composition than would be expected from neutral assembly processes. This variation did not translate into any clear partitioning into discrete community types, that is, distinct compositional states, such as enterotypes in the human gut. However, a strong partition into community types did emerge at the strain level for the dominant organism: genotypes of Candidatus Accumulibacter that coexisted in the metacommunity (the reactor) excluded each other within community replicates (granules). Individual granule communities maintained a significant lineage structure, whereby the strain phylogeny of Accumulibacter correlated with the overall composition of the community, indicating a high potential for co-diversification among species and communities. Our results suggest that due to the high functional redundancy and competition between close relatives, alternative community types are most probably observed at the level of recently differentiated genotypes but not at higher orders of genetic resolution.en_US
dc.description.sponsorshipSwiss National Science Foundation (Grant 162251)en_US
dc.description.sponsorshipHuman Frontier Science Program (Strasbourg, France) (Grant LT000643/2016-L)en_US
dc.description.sponsorshipSimons Foundation (Grant 542395)en_US
dc.language.isoen
dc.publisherSpringer Natureen_US
dc.relation.isversionofhttps://dx.doi.org/10.1038/S41564-018-0242-3en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcebioRxiven_US
dc.titleStrain-level diversity drives alternative community types in millimetre-scale granular biofilmsen_US
dc.typeArticleen_US
dc.identifier.citationLeventhal, Gabriel E. et al. “Strain-level diversity drives alternative community types in millimetre-scale granular biofilms” Nature Microbiology, vol. 3, no. 11, 2018, pp. 1295-1303.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Civil and Environmental Engineeringen_US
dc.relation.journalNature Microbiologyen_US
dc.eprint.versionOriginal manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/NonPeerRevieweden_US
dc.date.updated2020-05-21T14:28:59Z
dspace.date.submission2020-05-21T14:29:04Z
mit.journal.volume3en_US
mit.journal.issue11en_US
mit.licensePUBLISHER_POLICY


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