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dc.contributor.authorLee, Hyewon
dc.contributor.authorLee, Jiseok
dc.contributor.authorDoyle, Patrick S
dc.date.accessioned2020-06-05T15:08:59Z
dc.date.available2020-06-05T15:08:59Z
dc.date.issued2020-03
dc.identifier.issn1520-6882
dc.identifier.urihttps://hdl.handle.net/1721.1/125688
dc.description.abstractAlthough microRNA (miRNA) expression levels provide important information regarding disease states owing to their unique dysregulation patterns in tissues, translation of miRNA diagnostics into point-of-care (POC) settings has been limited by practical challenges. Here, we developed a hydrogel-based microfluidic platform for colorimetric profiling of miRNAs, without the use of complex external equipment for fluidics and imaging. For sensitive and reliable measurement without the risk of sequence bias, we employed a gold deposition-based signal amplification scheme and dark-field imaging, and seamlessly integrated a previously developed miRNA assay scheme into this platform. The assay demonstrated a limit of detection of 260 fM, along with multiplexing of small panels of miRNAs in healthy and cancer samples. We anticipate this versatile platform to facilitate a broad range of POC profiling of miRNAs in cancer-associated dysregulation with high-confidence by exploiting the unique features of hydrogel substrate in an on-chip format and colorimetric analysis.en_US
dc.description.sponsorshipNational Institute of Biomedical Imaging and Bioengineering (Grant 5R21EB024101-02)en_US
dc.description.sponsorshipKorea (South). Ministry of Science, ICT and Future Planning (Grant 2018M3D3A1A01055732)en_US
dc.description.sponsorshipNational Research Foundation of Korea (Grant 2018R1C1B6004086)en_US
dc.language.isoen
dc.publisherAmerican Chemical Society (ACS)en_US
dc.relation.isversionofhttps://dx.doi.org/10.1021/acs.analchem.9b05043en_US
dc.rightsCreative Commons Attribution 4.0 International licenseen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.sourceACSen_US
dc.titleHydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Deviceen_US
dc.typeArticleen_US
dc.identifier.citationLee, Hyewon et al. “Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device” Analytical Chemistry, vol. 92, no. 8, 2020, pp. 5750-5755 © 2020 The Author(s)en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.relation.journalAnalytical Chemistryen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2020-05-18T16:34:28Z
dspace.date.submission2020-05-18T16:34:30Z
mit.journal.volume92en_US
mit.journal.issue8en_US
mit.licensePUBLISHER_CC


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