Microneedle‐based intradermal delivery of stabilized dengue virus
Author(s)Turvey, Michelle E.; Uppu, Divakara S.S.M.; Mohamed Sharif, Abdul Rahim; Bidet, Katell; Alonso, Sylvie; Ooi, Eng Eong; Hammond, Paula T; ... Show more Show less
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Current live-attenuated dengue vaccines require strict cold chain storage. Methods to preserve dengue virus (DENV) viability, which enable vaccines to be transported and administered at ambient temperatures, will be decisive towards the implementation of affordable global vaccination schemes with broad immunization coverage in resource-limited areas. We have developed a microneedle (MN)-based vaccine platform for the stabilization and intradermal delivery of live DENV from minimally invasive skin patches. Dengue virus-stabilized microneedle arrays (VSMN) were fabricated using saccharide-based formulation of virus and could be stored dry at ambient temperature up to 3 weeks with maintained virus viability. Following intradermal vaccination, VSMN-delivered DENV was shown to elicit strong neutralizing antibody responses and protection from viral challenge, comparable to that of the conventional liquid vaccine administered subcutaneously. This work supports the potential for MN-based dengue vaccine technology and the progression towards cold chain-independence. Dengue virus can be stabilized using saccharide-based formulations and coated on microneedle array vaccine patches for storage in dry state with preserved viability at ambient temperature (VSMN; virus-stabilized microneedle arrays). Keywords: dengue; immunization cold chain; microneedles; vaccine delivery
DepartmentSingapore-MIT Alliance in Research and Technology (SMART); David H. Koch Institute for Integrative Cancer Research at MIT; Massachusetts Institute of Technology. Department of Chemical Engineering
Bioengineering & Translational Medicine
Turvey, Michelle E. et al., "Microneedle‐based intradermal delivery of stabilized dengue virus." Bioengineering & Translational Medicine 4, 2 (May 2019): e10127 © 2019 The Author(s).
Final published version