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dc.contributor.authorChen, Guan-Yu
dc.contributor.authorLi, Zeyang
dc.contributor.authorDuarte, Joao N.
dc.contributor.authorEsteban, Alexandre
dc.contributor.authorCheloha, Ross W.
dc.contributor.authorTheile, Christopher S
dc.contributor.authorFink, Gerald R.
dc.contributor.authorPloegh, Hidde
dc.date.accessioned2020-06-12T14:33:59Z
dc.date.available2020-06-12T14:33:59Z
dc.date.issued2017-03
dc.date.submitted2016-10
dc.identifier.issn0956-5663
dc.identifier.issn0265-928X
dc.identifier.urihttps://hdl.handle.net/1721.1/125780
dc.description.abstractCurrent techniques to characterize leukocyte subgroups in blood require long sample preparation times and sizable sample volumes. A simplified method for leukocyte characterization using smaller blood volumes would thus be useful in diagnostic settings. Here we describe a flow system comprised of two functionalized graphene oxide (GO) surfaces that allow the capture of distinct leukocyte populations from small volumes blood using camelid single-domain antibodyfragments (VHHs) as capture agents. We used site-specifically labeled leukocytes to detect and identify cells exposed to fungal challenge. Combining the chemical and optical properties of GO with the versatility of the VHH scaffold in the context of a flow system provides a quick and efficient method for the capture and characterization of functional leukocytes. Keywords: Single domain antibody, Graphene oxide, Sortase, Enzymatic labeling, Cell detection, Leukocytesen_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (NIH Grant no. 4DP1GM106409)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (NIH Grant no. R01AI87879)en_US
dc.description.sponsorshipTaiwan. Ministry of Science and Technology (MOST 105-2633-B-009-003)en_US
dc.description.sponsorshipTaiwan. Ministry of Science and Technology (MOST 105-2628-B-009-001-MY3)en_US
dc.description.sponsorshipNational Chiao Tung University (Taiwan) (105W970)en_US
dc.language.isoen
dc.publisherElsevier BVen_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/J.BIOS.2016.10.015en_US
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs Licenseen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.sourcePMCen_US
dc.titleRapid Capture and Labeling of Cells on Single Domain Antibodies-Functionalized Flow Cellen_US
dc.typeArticleen_US
dc.identifier.citationChen, Guan-Yu, Zeyang Li, Joao N. Duarte et al. "Rapid Capture and Labeling of Cells on Single Domain Antibodies-Functionalized Flow Cell" Biosensors and Bioelectronics 89,2 (March 2017):789-794. © 2016 Elsevier B.V.en_US
dc.contributor.departmentWhitehead Institute for Biomedical Researchen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.relation.journalBiosensors and Bioelectronicsen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2019-09-18T12:47:55Z
dspace.orderedauthorsChen, Guan-Yu; Li, Zeyang; Duarte, Joao N.; Esteban, Alexandre; Cheloha, Ross W.; Theile, Christopher S.; Fink, Gerald R.; Ploegh, Hidde L.en_US
dspace.date.submission2019-09-18T12:47:56Z
mit.journal.volume89en_US
mit.journal.issue2en_US


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