Stable, small, specific, low-valency quantum dots for single-molecule imaging
Author(s)
Lee, Jungmin; Feng, Xinyi; Chen, Ou; Bawendi, Moungi G; Huang, Jun
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We have developed a strategy for synthesizing immediately activable, water-soluble, compact (∼10–12 nm hydrodynamic diameter) quantum dots with a small number of stable and controllable conjugation handles for long distance delivery and subsequent biomolecule conjugation. Upon covalent conjugation with engineered monovalent streptavidin, the sample results in a population consisting of low-valency quantum dots. Alternatively, we have synthesized quantum dots with a small number of biotin molecules that can self-assemble with engineered divalent streptavidin via high-affinity biotin–streptavidin interactions. Being compact, stable and highly specific against biotinylated proteins of interest, these low-valency quantum dots are ideal for labeling and tracking single molecules on the cell surface with high spatiotemporal resolution for different biological systems and applications.
Date issued
2018-01Department
Massachusetts Institute of Technology. Department of ChemistryJournal
Nanoscale
Publisher
Royal Society of Chemistry (RSC)
Citation
Lee, Jungmin et al. "Stable, small, specific, low-valency quantum dots for single-molecule imaging." Nanoscale 10, 9 (January 2018): 4406-4414
Version: Author's final manuscript
ISSN
2040-3364
2040-3372