Stable, small, specific, low-valency quantum dots for single-molecule imaging
Author(s)Lee, Jungmin; Feng, Xinyi; Chen, Ou; Bawendi, Moungi G; Huang, Jun
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We have developed a strategy for synthesizing immediately activable, water-soluble, compact (∼10–12 nm hydrodynamic diameter) quantum dots with a small number of stable and controllable conjugation handles for long distance delivery and subsequent biomolecule conjugation. Upon covalent conjugation with engineered monovalent streptavidin, the sample results in a population consisting of low-valency quantum dots. Alternatively, we have synthesized quantum dots with a small number of biotin molecules that can self-assemble with engineered divalent streptavidin via high-affinity biotin–streptavidin interactions. Being compact, stable and highly specific against biotinylated proteins of interest, these low-valency quantum dots are ideal for labeling and tracking single molecules on the cell surface with high spatiotemporal resolution for different biological systems and applications.
DepartmentMassachusetts Institute of Technology. Department of Chemistry
Royal Society of Chemistry (RSC)
Lee, Jungmin et al. "Stable, small, specific, low-valency quantum dots for single-molecule imaging." Nanoscale 10, 9 (January 2018): 4406-4414
Author's final manuscript