A CRISPR-based assay for the detection of opportunistic infections post-transplantation and for the monitoring of transplant rejection
Author(s)
Kaminski, Michael M.; Alcantar, Miguel A.; Lape, Isadora T.; Greensmith, Robert; Huske, Allison C.; Valeri, Jacqueline A.; Marty, Francisco M.; Klämbt, Verena; Azzi, Jamil; Akalin, Enver; Riella, Leonardo V.; Collins, James J.; ... Show more Show less![Thumbnail](/bitstream/handle/1721.1/125918/200622_nBME_CrisprDx.pdf.jpg?sequence=4&isAllowed=y)
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In organ transplantation, infection and rejection are major causes of graft loss. They are linked by the net state of immunosuppression. To diagnose and treat these conditions earlier, and to improve long-term patient outcomes, refined strategies for the monitoring of patients after graft transplantation are needed. Here, we show that a fast and inexpensive assay based on CRISPR–Cas13 accurately detects BK polyomavirus DNA and cytomegalovirus DNA from patient-derived blood and urine samples, as well as CXCL9 messenger RNA (a marker of graft rejection) at elevated levels in urine samples from patients experiencing acute kidney transplant rejection. The assay, which we adapted for lateral-flow readout, enables—via simple visualization—the post-transplantation monitoring of common opportunistic viral infections and of graft rejection, and should facilitate point-of-care post-transplantation monitoring.
Date issued
2020-04Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science; Broad Institute of MIT and HarvardJournal
Nature Biomedical Engineering
Publisher
Springer Science and Business Media LLC
Citation
Kaminski, Michael M. et al. "A CRISPR-based assay for the detection of opportunistic infections post-transplantation and for the monitoring of transplant rejection." Nature Biomedical Engineering 4, 6 (April 2020): 601–609 © 2020 The Author(s)
Version: Author's final manuscript
ISSN
2157-846X