Show simple item record

dc.contributor.authorEnglish, Max A.
dc.contributor.authorSoenksen Martinez, Luis Ruben
dc.contributor.authorGayet, Raphael V.
dc.contributor.authorde Puig Guixe, Helena
dc.contributor.authorAngenent-Mari, Nicolaas M.
dc.contributor.authorMao, Angelo S.
dc.contributor.authorNguyen, Peter Q.
dc.contributor.authorCollins, James J.
dc.date.accessioned2020-06-22T18:59:20Z
dc.date.available2020-06-22T18:59:20Z
dc.date.issued2019-08
dc.date.submitted2019-01
dc.identifier.issn0036-8075
dc.identifier.issn1095-9203
dc.identifier.urihttps://hdl.handle.net/1721.1/125920
dc.description.abstractStimuli-responsive materials activated by biological signals play an increasingly important role in biotechnology applications. We exploit the programmability of CRISPR-associated nucleases to actuate hydrogels containing DNA as a structural element or as an anchor for pendant groups. After activation by guide RNA–defined inputs, Cas12a cleaves DNA in the gels, thereby converting biological information into changes in material properties. We report four applications: (i) branched poly(ethylene glycol) hydrogels releasing DNA-anchored compounds, (ii) degradable polyacrylamide-DNA hydrogels encapsulating nanoparticles and live cells, (iii) conductive carbon-black–DNA hydrogels acting as degradable electrical fuses, and (iv) a polyacrylamide-DNA hydrogel operating as a fluidic valve with an electrical readout for remote signaling. These materials allow for a range of in vitro applications in tissue engineering, bioelectronics, and diagnostics.en_US
dc.description.sponsorshipDefense Threat Reduction Agency (Grant HDTRA1-14-1-0006)en_US
dc.language.isoen
dc.publisherAmerican Association for the Advancement of Science (AAAS)en_US
dc.relation.isversionofhttp://dx.doi.org/10.1126/science.aaw5122en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourceProf. Collins via Howard Silveren_US
dc.titleProgrammable CRISPR-responsive smart materialsen_US
dc.typeArticleen_US
dc.identifier.citationEnglish, Max A. et al. " Programmable CRISPR-responsive smart materials." Science 365, 6455 (August 2019): 780-785 © 2017 The Authorsen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Institute for Medical Engineering and Scienceen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Synthetic Biology Centeren_US
dc.relation.journalScienceen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2020-06-22T14:27:49Z
dspace.date.submission2020-06-22T14:27:52Z
mit.journal.volume365en_US
mit.journal.issue6455en_US
mit.licenseOPEN_ACCESS_POLICY


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record